摘要
目的探讨病毒性心肌炎患儿血清高迁移率蛋白-1(HMGB-1)和血管内皮细胞钙黏蛋白(VE-cadherin)的变化及临床意义。方法采用ELISA法测定52例病毒性心肌炎患儿血清HMGB-1和VE-cadherin含量,同时检测磷酸肌酸激酶同工酶(CK—MB),并与36例健康儿童作对照。结果病毒性心肌炎患儿血清HMGB1、VE—cadherin和CK-MB急性期含量[HMGB1(5.14±0.23)mg/L;VE—cadhefin(5.36±0.92)ms/L;CK—MB(31.42±3.22)U/L]明显高于恢复期[HMGB1(0.92±0.14)mg/L;VE-cadherin(2.93±0.64)mg/L;CK.MB(13.75±3.18)U/L](t值分别为11.37、10.26、12.17,P均〈0.01)及对照组[HMGB1(0.86±0.12)mg/L;VE—cadherin(2.86±0.65)mg/L;CK-MB(12.83±3.04)U/L](t值分别为12.06、11.19、12.64,P均〈0.01);恢复期HMGBI、VE—cadhefin和CK—MB水平与对照组比较,差异均无统计学意义(t值分别为1.26、1.19、1.43,P均〉0.05)。病毒性心肌炎患儿HMGB1与VE—cadherin、CK-MB呈正相关(r值分别为0.73、0.79,P均〈0.05),VE:cadherin与CK-MB亦呈正相关(r=0.82,P〈0.05)。结论HMGB1和VE—cadhefin可能参与病毒性心肌炎的发生发展,并可作为病情判断及预后的观察指标。
Objective To investigate the changes of high mobility group box-1 (HMGB1) and VE- cadherin in serum of children with viral myocarditis and their clinical significance. Methods The serum levels of HMCB1 and VE-cadherin were detected by ELISA in 52 children with viral myocarditis, and 36 normal healthy children were enfolded as control. CK-MB was also measured in all subjects enrolled into the study. Results The serum levels of HMGB1, VE-cadherin and CK-MB in children with acute stage Viral myocarditis (HMGB1 : [5. 14 ±0. 23 ] mg,/L; VE-cadherin: [5.36±0. 92] mg/L;CK-MB : [31.42 ±3.22] U/L) were significantly higher than those with recovery stage viral myocarditis ( HMGB1 : [ 0. 92± 0. 14 ] rag/L, VE- cadherin: [2. 93 ±0. 64] mg/L; CK-MB: [ 13.75± 3.18 ] U/L) ( t = 11.37,10. 26 and 12. 17 respectively , Ps 〈 0. 01 ) and control ( HMGB1 : [ 0. 86± 0. 12 ] mg/L; VE-cadherin: [ 2. 86 ± 0. 65 ] mg/L; CK-MB : [ 12. 83 ± 3. 04 ] U/L) ( t = 12. 06,11.19 and 12. 64 respectively, Ps 〈 0. 01 ). However, we found no significant difference in the serum levels of HMGB1, VE-cadherin and CK-MB between recovery stage viral myocarditis group and the control (t = 1.26,1.19,1.43 ,Ps 〉 0. 05 ). There were positive correlations between HMGB1 and VE-cadherin, CK-MB (r =0.73,0. 79 ,Ps 〈0. 05 ) ; and positive correlation between VE-cadherin and CK-MB ( r = 0. 82 ,P 〈 0. 05). Conclusion HMGB1 and VE-cadherin may play roles in the viral myocarditis pathogenesis,which can be new prognosis factors for viral myocarditis.
出处
《中国综合临床》
2011年第8期880-883,共4页
Clinical Medicine of China