病毒性心肌炎患儿血清高迁移率蛋白-1和血管内皮细胞钙黏蛋白的变化

Changes of HMGB1 and VE-cadherin in serum of children with viral myocarditis

目的探讨病毒性心肌炎患儿血清高迁移率蛋白-1（HMGB-1）和血管内皮细胞钙黏蛋白（VE-cadherin）的变化及临床意义。方法采用ELISA法测定52例病毒性心肌炎患儿血清HMGB-1和VE-cadherin含量，同时检测磷酸肌酸激酶同工酶（CK—MB），并与36例健康儿童作对照。结果病毒性心肌炎患儿血清HMGB1、VE—cadherin和CK-MB急性期含量[HMGB1（5．14±0．23）mg／L；VE—cadhefin（5．36±0．92）ms／L；CK—MB（31．42±3．22）U／L]明显高于恢复期[HMGB1（0．92±0．14）mg／L；VE-cadherin（2．93±0．64）mg／L；CK．MB（13．75±3．18）U／L]（t值分别为11．37、10．26、12．17，P均〈0.01）及对照组[HMGB1（0．86±0．12）mg/L；VE—cadherin（2．86±0．65）mg／L；CK-MB（12．83±3．04）U／L]（t值分别为12．06、11．19、12．64，P均〈0．01）；恢复期HMGBI、VE—cadhefin和CK—MB水平与对照组比较，差异均无统计学意义（t值分别为1．26、1．19、1．43，P均〉0．05）。病毒性心肌炎患儿HMGB1与VE—cadherin、CK-MB呈正相关（r值分别为0．73、0．79，P均〈0．05），VE：cadherin与CK-MB亦呈正相关（r=0．82，P〈0．05）。结论HMGB1和VE—cadhefin可能参与病毒性心肌炎的发生发展，并可作为病情判断及预后的观察指标。

Objective To investigate the changes of high mobility group box-1 （HMGB1） and VE- cadherin in serum of children with viral myocarditis and their clinical significance. Methods The serum levels of HMCB1 and VE-cadherin were detected by ELISA in 52 children with viral myocarditis, and 36 normal healthy children were enfolded as control. CK-MB was also measured in all subjects enrolled into the study. Results The serum levels of HMGB1, VE-cadherin and CK-MB in children with acute stage Viral myocarditis （HMGB1 ： [5. 14 ±0. 23 ] mg,/L; VE-cadherin： [5.36±0. 92] mg/L;CK-MB ： [31.42 ±3.22] U/L） were significantly higher than those with recovery stage viral myocarditis （ HMGB1 ： [ 0. 92± 0. 14 ] rag/L, VE- cadherin： [2. 93 ±0. 64] mg/L; CK-MB： [ 13.75± 3.18 ] U/L） （ t = 11.37,10. 26 and 12. 17 respectively , Ps 〈 0. 01 ） and control （ HMGB1 ： [ 0. 86± 0. 12 ] mg/L; VE-cadherin： [ 2. 86 ± 0. 65 ] mg/L; CK-MB ： [ 12. 83 ± 3. 04 ] U/L） （ t = 12. 06,11.19 and 12. 64 respectively, Ps 〈 0. 01 ）. However, we found no significant difference in the serum levels of HMGB1, VE-cadherin and CK-MB between recovery stage viral myocarditis group and the control （t = 1.26,1.19,1.43 ,Ps 〉 0. 05 ）. There were positive correlations between HMGB1 and VE-cadherin, CK-MB （r =0.73,0. 79 ,Ps 〈0. 05 ） ; and positive correlation between VE-cadherin and CK-MB （ r = 0. 82 ,P 〈 0. 05）. Conclusion HMGB1 and VE-cadherin may play roles in the viral myocarditis pathogenesis,which can be new prognosis factors for viral myocarditis.