牛Gtl2基因cDNA序列分析及启动子预测

Sequencing analysis of bovine Gtl2 cDNA and promoter prediction

利用RT-PCR和RACE方法,克隆得到了牛Gtl2基因cDNA全序列,生物信息学分析表明:该序列有8个外显子,含有一个最长645 bp的开放读码框,但没有发现与Kozak规则相匹配的翻译起始序列。Gtl2基因在物种间具有保守性,尤其是第1个外显子表现出了较强的保守性。基因进化树分析表明,牛Gtl2基因与绵羊Gtl2基因的亲缘关系最近。使用启动子预测,在线软件对牛Gtl2基因的5′侧翼序列进行分析,得出了潜在的启动子区域,这些区域含有TATA,CAAT框和GC框,并存在一些转录因子结合位点,而且在5′侧翼区域发现有3个CpG岛。转录因子结合位点和CpG岛可能与牛Gtl2基因的转录及其表达调控有关。

The full length cDNA of bovine Gtl2 gene was obtained by using the method of RT-PCR and RACE.The bioinformatic analysis revealed that 8 exons existed in the Gtl2 gene and the longest opening reading frame（ORF）of this gene is 645 bp.However,none of the ATGs is consistent with Kozak consensus sequence.Gtl2 gene is conserved among species,especially for exon 1.Phylogenetic tree analysis showed that the bovine Gtl2 gene shares high identity to the sheep homology.Then the potential promoter was predicted with TATA-box,CAAT-box and GC box in 5′-flanking sequence.The 5′-flanking region contained many potential transcriptional factor binding sites and three CpG islands which might have an important effect on transcription activation and expression regulation.