不同配比乌头汤组方中两种生物碱含量变化

Contents Study of Two Alkloids in Wutou Decoction with Different Proportion

目的研究乌头汤不同配比组方中新乌头碱和次乌头碱含量的变化。方法采用高效液相色谱法,将乌头汤中制川乌与其余各药以不同量配比,考察对新乌头碱、次乌头碱的影响。结果所建立的新乌头碱、次乌头碱线性回归方程分别为Y=4.253X+30.98(r=0.999 4),Y=3.713X+96.02(r=0.999 3);线性范围分别为4.95～79.20,12.18～194.80μg.L-1。乌头汤中制川乌与其余各药不同配比对新乌头碱和次乌头碱含量影响很大,当制川乌、麻黄、黄芪、白芍和炙甘草组方为65,1,37,1,3 g时,新乌头碱和次乌头碱含量之和最大。新乌头碱和次乌头碱在线性范围内线性关系良好。结论以新乌头碱和次乌头碱含量变化研究乌头汤组方配比,能更好地保证乌头汤的疗效,降低其毒副作用。

Objective To investigate the contents of mesaconiatine and hypaconitine in wutou decoction with different proportion. Methods Aconiti Radix Cocta was combined with other herbs in different ratio, and the content of mesaconiatine and hypaconitine in which was investigated by HPLC. Results For mesaconiatine and hypaconitine, the linear regression equation were set as Y= 4. 253X＋30.98 （ r = 0. 999 4） and Y= 3. 713X＋96.02 （ r = 0. 999 3 ）, with the linear range of 4.95- 79.20 μg· L^-1 and 12.18-194.80μg·L^-1 , respectively. It presented a great influence on the contents of mesaconiatine and hypaconitine by the prepared Aconiti Radix being combined with other herbs at different ratio. The best wutou decoction composed of 6 g prepared Radix Aconiti, 5 g Ephedra, 13 g Astragali Radix, 7 g Paeoaiae Radix Alba, 13 g prepared Radix Glycyrrhizae, which leading to the optimum contents of mesaconiatine and hypaconitine. The linear relation of mesaconiatine and hypaconitine was good within their linear range. Conclusion The investigation on the combinations of watou decoction based on the content change of mesaconiatine and hypaconitine can ensure the efficacy and reduce the toxicity of wutou decoction.