火焰原子吸收光谱法测定大黄牡丹汤中11种元素含量

Content Determination of 11 Metal Elements in Decoction of Rheum and Moutan by Flame Atomic Absorption Spectrometry

目的测定大黄牡丹汤中钙(Ca)、铜(Cu)、铁(Fe)、钾(K)、镁(Mg)、镍(Ni)、铬(Cr)、钼(Mo)、锌(Zn)、镉(Cd)和钠(Na)等11种元素含量。方法采用空气-乙炔火焰原子吸收光谱法,硝酸-高氯酸(4∶1)常压微沸条件下消解样品,测定大黄牡丹汤中11种元素并进行方法准确度和精密度考察。结果大黄牡丹汤中11种元素含量RSD为0.68%～2.00%,加样回收率为96.5%～99.8%。结论该方法简单、快速,适合大黄牡丹汤中Ca、Cu、Fe、K、Mg、Ni、Cr、Mo、Zn、Cd、Na的含量测定,同时为大黄牡丹汤的临床疗效研究提供了有效依据。

Objective Determine the content of 11 metal elements （ Ca, Cu, Fe, K, Mg, Ni, Cr, Mo, Zn, Cd, Na） in decoction of rheum and moutan. Methods The air-acetylene flame atomic absorption spectrometry was used to determine the elements in decoction of rheum and moutan and the accuracy and precision of which were studied. The samples were digested with HNO3-HCIO4（4 ： 1 ） at 140 ℃ under normal pressure. Results The RSD of 11 metal elements and recoveries were 0.68% 2.00% and 96.8% - 99.7%, respectively. Conclusion The method is simple,rapid and appropriate for content determining of the 11 metal elements, which providing an effective basis for evaluating clinical efficacy of Rhemn and Moutan decoction.