红外光谱监测糖化酶发酵过程

Monitoring Glucoamylase Fermentation with Infrared Spectroscopy

傅里叶变换红外光谱用于监测糖化酶发酵过程， 培养液的红外光谱反映了培养过程中碳源和氮源的变化。针对培养过程中普遍使用复合培养基， 标准混合溶液难以反映培养液的状态。用偏最小二乘法建立了培养液的红外光谱数据与常规离线化学分析结果的相关关系， 成功地用于糖化酶发酵过程培养液中总糖、还原糖和氨氮的预测， 测定结果与离线化学分析方法的相关性分别为０．９９９， ０．９９０和０．９７４。工业生产过程的菌种、培养基和工艺条件相对稳定，

The glucoamylase fermentation was investigated with Fourier transform infrared spectroscopy.During the cultivation,the variation of carbon and nitrogen source can be reflected from the infrared spectra.In most cases,the complex media are used to cultivate cells or bacteria.It is difficult to construct calibration samples with synthetic mixtures which can represent the composition of real broth.The infrared spectral data of the real broth and off line chemical analysis results were used to establish the calibration model.The model is successfully used to predict the concentrations of total sugar,reduced sugar,and nitrogen in real broth.The correlation coefficients between the infrared analysis results and chemical analysis are 0 999,0 990 and 0 974 for total sugar,reduced sugar and nitrogen,respectively.The bacteria,culture and operating condition are relatively stable during the practical productive processes.The method can be used to monitor the industrial processes.