蛹虫草小麦培养基中虫草素提取工艺研究

Extraction Protocol of Cordycepin from Cordyceps Wheat Medium

通过对比提取溶剂、料液比、温度、pH值及时间对提取蛹虫草小麦培养基中虫草素的影响,以确定虫草素提取最佳工艺参数。结果表明:最佳提取参数为水提取、pH值5,料液比1∶50、温度70℃、时间3h。该方法从蛹虫草小麦培养基中提取虫草素,提取率可达94.87%。

To maximize the extraction efficiency of cordycepin from Cordyceps, the extraction procedure was optimized via modifying wheat medium including different extraction solution, rate of mater/al to solvent, temperature, pH and time. The result revealed that the optimum extraetion parameters were as follows： water as extraetion solution, pH 5, ratio of material to liquid 1： 50, 70℃, and 3 h, by which cordycepin might be extracted from Cordyceps conveniently and efficiently, and the extraction rate was as high as 94.87%.