microRNA-7对人肺癌95D细胞体外侵袭的作用

The effects of microRNA-7 on the invasion of human lung cancer cell line 95D cells in vitro

目的探讨微小RNA-7(microRNA-7,miR-7)对人肺癌95D细胞体外侵袭的作用。方法采用体外转染法将miR-7瞬时转染95D细胞后,应用Real-timePCR特异探针法检测95D细胞中miR-7的表达情况,应用划痕法检测95D细胞的迁移情况,Invasion实验检测95D细胞侵袭能力的变化;用Western印迹法检测95D细胞中胰岛素样生长因子1受体(Insulin growth factor 1 receptor,IGF1R)表达及下游信号的变化。结果与对照组相比,miR-7转染组95D细胞体外侵袭能力明显削弱(<0.05),而IGF1R表达则显著下调,其信号途径分子Akt的磷酸化水平也明显降低。结论 miR-7可以抑制人肺癌95D细胞的体外侵袭,可能作为后续肺癌生物治疗的新靶点。

Objective To explore the effects of miRNA-7 on the invasion of human lung cancer cell line 95D cells in vitro.Methods miRNA-7 was transiently transferred into 95D cells using FuGENER-HD reagent.The expression level of miRNA-7 on 95D cells transferred with miRNA-7 was determined by Real-time PCR using specific probe before or after transfection.The migration of 95D cells transferred with miRNA-7 was accessed by Scratch assay.Meanwhile,the invasion capacity of 95D cells was accessed by in vitro invasion model.The expression of IGF1R and phosphorylation of Akt also were determined by western blot.Results The expression level of miRNA-7 on 95D cells transferred with miRNA-7 significantly increased compared with that in cont groups（P0.05）.The migration and invasion capacity of 95D cells transferred with miRNA-7 markedly decreased（P0.05）.Moreover,miRNA-7 potently inhibited the expression of IGF1R on 95D cells,as well as reduced the phosphorylation of Akt（P0.05）.Conclusions miRNA-7 could inhibit the invasion capacity of 95D cells,suggesting that miRNA-7 might be a novel target for the biological therapy of human lung cancer.