摘要
根据GenBank中发表的犬瘟热病毒Onderstepoort株N蛋白基因序列设计两对特异性引物,采用RT-PCR扩增犬瘟热病毒贵州分离株(CDV-GZ1)的N基因,并进行克隆与序列分析。结果显示,CDV-GZ1株N基因的ORF全长1572bp,其编码氨基酸序列与国外Shuskiy株和01-2689株的同源性分别为98.9%和97.1%,与部分国内分离株同源性在95%以上,说明N蛋白是保守性较强的结构蛋白。
Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank,and the N gene was amplified by reverse transcription polymerase chain reaction(RT-PCR) from CDV-GZ1 strain in Guizhou.The amplified fragment was cloned and analyzed.The results showed that the length of N gene was 1572 bp.The homology of coding amino acid between this strain and CDV Shuskiy and 01-2689 strain was 98.9% and 97.1% respectively,while above 95% among other domestic strain.It illustrated that N protein have highly conservation property.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第8期63-66,共4页
China Animal Husbandry & Veterinary Medicine
基金
贵州大学博士基金(X060054)
关键词
犬瘟热病毒
N蛋白
克隆
序列分析
canine distemper virus
N protein
clone
sequence analysis