鹅大肠杆菌油佐剂灭活疫苗的研制

Development of Oil Adjuvant Inactivated Vaccine against Pathogenic Escherichia coli of Geese

从湛江地区疑似大肠杆菌病的病死鹅组织中分离鉴定2株致病性大肠杆菌O86K61和O44K74,以此制备油佐剂灭活疫苗。用上述疫苗接种20日龄雏鹅,35日龄加强免疫一次,分别于27、35、42、50日龄检测免疫鹅血清抗体水平,并进行攻毒保护试验。结果表明,制备的大肠杆菌油佐剂灭活疫苗安全可靠,免疫后15、30d后,免疫鹅血清抗体水平分别达到1∶32和1∶128;且免疫鹅能抵抗1010/mL的致病性大肠杆菌攻击,对照组鹅全部发病,有明显临床症状,从发病鹅组织中分离到致病性大肠杆菌。

2 pathogenic Escherichia coli strains O86K61 and O44K74 were isolated from geese colibacillosis suspect cases in Zhanjiang area,and were used to produce oil adjuvant inactivated vaccine.20-day-old geese were inoculated with the vaccine and boosted 15 days late.The blood samples were collected and the serum agglutination antibody titers of the geese were determined to evaluate the immune efficiency,then the geese were challenged with 1010/mL pathogenic Escherichia coli to confirm the immune protection effect of the emulsion vaccine.The results showed that the vaccine above was safe and reliable,the serum antibody titers of the immunized birds came to 1∶32 and 1∶128 respectively inoculated 15 and 30 days late.Moreover,the vaccine inoculated birds were all protected against the challenge of the pathogenic bacteria,whereas the control animals exhibited visible clinical signs of colibacillosis,and the pathogenic Escherichia coli O86 and O44 subtypes could be isolated from the sick geese.