摘要
为探索H-FABP和LPIN1基因在不同猪种中的多态性分布情况,应用PCR-RFLP技术检测了61头军牧1号白猪、51头杜洛克猪和51头藏猪H-FABP和LPIN1基因的多态性分布。结果发现,军牧1号白猪、杜洛克猪和藏猪在H-FABP基因的HinfⅠ多态性位点上均表现为单一的HH型,而HeaⅢ多态性位点上均表现出多态性,等位基因D的基因频率分别为0.1475、0.2255和0.7647,MspⅠ多态位点上,军牧1号白猪表现为单一的aa型,杜洛克猪和藏猪则表现为多态性,等位基因A的基因频率分别为0.6078和0.4609。在LPIN1基因的Eco88Ⅰ多态性位点上,杜洛克猪表现为单一的AA型,军牧1号白猪和藏猪表现为多态性,等位基因A的基因频率分别为0.4262和0.2059;在Bsh1236Ⅰ多态性位点上,杜洛克猪表现为单一的TT型,军牧1号白猪和藏猪则表现为多态性,等位基因T的基因频率分别为0.7459和0.2059。
In order to observe the polymorphism distribution of H-FABP and LPIN1 gene in different breeds of swines,analysis was carried out upon 61 Junmu 1 White pigs,51 Durocs pigs and 51 Tibetian pigs by PCR-RFLP.The results revealed that all these three breeds showed a single genotype of HH on the Hinf Ⅰ locus and showed polymorphism on the Hae Ⅲ locus for H-FABP,with D allelic frequency of 0.1475,0.2255 and 0.7647,respectively.The H-FABP/Msp Ⅰ locus,however,behaved a single genotype of aa in Junmu 1 White pigs and the A allelic frequency was 0.6078 and 0.4609 in Duroc and Tibetian pigs respectively.For LPIN1 /Eco88 Ⅰ,Duroc pigs showed single genotype of AA and Junmu 1 White and Tibetian pigs showed polymorphism with A allelic frequency of 0.4262 and 0.2059,respectively.For LPIN1/Bsh1236 Ⅰ locus,Duroc pigs showed single genotype of TT,Junmu 1 White and Tibetian pigs showed polymorphism with the T allelic frequency of 0.7459 and 0.2059,respectively.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第8期141-146,共6页
China Animal Husbandry & Veterinary Medicine
基金
吉林省科技厅资助项目(20080208)
