海藻糖、二甲乙酰胺对公猪精液冷冻保存效果的研究

Effect of Using Dimethylacetamide in Combination with Trehalose on Cryopreservation of Boar Spermatozoa

采用5%二甲乙酰胺(DMA)(V/V)完全替代甘油,比较乳糖、海藻糖对精液冷冻保存效果的影响。结果表明,海藻糖显著提高了冷冻——解冻后精子成活力(49.32%±1.52%)与顶体完整性(47.33%±1.16%)(P<0.05)。然后利用海藻糖替代乳糖,评价不同浓度的DMA对公猪精液冷冻保存的影响。结果表明,当DMA添加量为4%时,解冻后精子活率、成活力、顶体完整率分别为(45.17±0.56)%、(50.33±0.67)%、(48.30±1.44)%,均显著高于3%DMA、6%DMA添加组(P<0.05),精子活率显著高于5%DMA添加组(P<0.05),但精子成活力、顶体完整性与其差异不显著(P>0.05)。因此,当利用海藻糖作为冷冻保存基础稀释液,DMA最适添加量为4%。

Two experiments were conducted to evaluate the dimethylacetylamide（DMF） and trehalose in boar semen cryopreservation protocols.In experiment 1,DMF was added at 5% to the diluents instead of glycerol used to freeze boar semen completely,evaluated lactose and trehalose on the effect of sperm cryopreservation.The results showed that compared with the lactose,trehalose increased significantly add frozen-thawed sperm viability（49.32±1.52）% and acrosome integrity（47.33%±1.16%）（P0.05）.In exprement 2,with trehalose replaced lactose,evaluated different concentrations of DMA on the cryopreservation of boar semen.The results showed that when the DMA was added at 4% level,sperm motility,viability and acrosome integrity after thawing were（45.17±0.56）%,（50.33±0.67）%,（48.30±1.44）% respectively,and were superior to 3% DMA,6% DMA（P0.05）,sperm motility was significantly higher than 5% DMA added group（P0.05）,but the sperm vitality,acrosome integrity were not significant difference（P0.05）.In conclusion,these results showed that when the use of trehalose as the basis of dilution of cryopreservation,the volume fraction of 4% DMA showed a better effect on the quality of boar cryopreservation semen.