肝硬化患者血清一氧化氮检测及其临床意义

Nitricoxide liever in cirrhotic patients and its clinical signifficance.

目的 探讨一氧化氮（ＮＯ）与肝硬化、肝功能分级及肝硬化并发症的关系。方法 应用Ｇｒｉｅｓｓ 比色法检测５６ 例肝硬化病人及２０ 例正常人血清ＮＯ 代谢产物亚硝酸盐和硝酸盐（ＮＯ－２ ／ＮＯ－３ ） 水平。结果 肝硬化组ＮＯ－２ ／ＮＯ－３ 水平为（３１－４±２４－０）μｍｏｌ／Ｌ，较正常对照组（６－２±１－９）μｍｏｌ／Ｌ 明显升高， Ｐ＜ ０－００１ 。而且随肝功能Ｃｈｉｌｄ－ Ｐｕｇｈ 分级的增加而升高。Ｃｈｉｌｄ － Ｐｕｇｈ Ａ 级与对照组比较，前者较后高，但统计学分析差异无显著性， Ｐ＞０－０５ 。Ｂ级与Ａ 级、Ｂ级与Ｃ级比较差异非常显著， Ｐ＜ ０－００１。并发原发性腹膜炎、食道胃底静脉曲张破裂出血和肝病性低氧血症组分别为（６７－５±１５－５） 、（２２－７ ±６－５） 、（４７－６ ±２２－９）μｍｏｌ／Ｌ，比无上述并发症肝硬化组的（１２－５±５－２）μｍｏｌ／Ｌ明显升高，Ｐ＜ ０－０１ ～０－００１ 。结论 肝硬化时ＮＯ合成增加，并且与肝硬化病情严重程度及并发症的发生密切相关。检测血清ＮＯ－２ ／ＮＯ－３

Objective Toinvestigatethelevelofplasma Nitric Oxide(NO)to assessitsrole onleverfunctioninjury and the complication ofcirrhosisand the possiblerelation between them .Methods NOwere measuredin plasmasamplescol lected from 56livercirrhosispatients with and20 healthycontrolsby NOcolorometricassay.Results Higherplasmalevel of NOwere observedin patients with cirrhosisthanin normalcontrols( P< 0-001) .The higher Child- Pugh ,the higher plasma NOlevel.Plasma NOlevel were higher in patients with primary peritonitis and the hemorrhage of esophageal varicesand hypoxemia than in patients without complication( P < 0-01 ～0-001).Conclusion Theincrease of plasma NOisclosely associated with theseverity oflevercirrhosisandthecomplication oflevercirrhosis