磁珠富集法筛选西施舌微卫星序列

Microsatellite enrichment by magnetic beads in Coelomactra antiquate

采用生物素-磁珠吸附微卫星富集法(FIASCO)筛选西施舌(Coelomactra antiquata)微卫星分子标记。结果获得含有重复次数不少于5次的微卫星序列38个,其中完美型占68.4%,非完美型26.3%,混合型5.3%。除探针中使用的CA和GA 2种重复外,还得到ATTG、CGTG和TGTTG的重复序列。微卫星重复次数主要集中在5～46次,最高为70次。利用这些微卫星序列设计了14对引物。该研究筛选出的微卫星标记为进一步研究西施舌的遗传多样性提供帮助。

In this experiment,magnetic bead hybridization method was used to enrich the microsatellite loci in Coelomactra antiquate.As a result,38 microsatellites that repeated no less than 5 times were found.Among the 38 microsatellites,68.4% are perfect,23.7% are imperfect and the rest are compound（7.9%）.In addition to the biotin-labeled SRS（simple repetitive sequence） probe CA and GA,there are ATTG,TG,CGTG and TGTTG in the microsatellites.Microsatellite repeats mainly from 5 to 46 times,and the most repetitive number is 70.We designed 14 pairs of SSR-primers using these microsatellite sites,which will facilitate the studies on the genetic diversity of C.antiquata.