针刺对抑郁症睡眠障碍大鼠行为学和海马5-羟色胺受体表达水平的影响

Effects of Acupuncture on Behaviors and Expression of 5-Hydroxytryptamine Receptor in Hippocampus in Sleep Deprivation Depression Rats

目的观察针刺治疗抑郁症睡眠障碍(SDD)的分子机制。方法将Open-Field法评分相近的36只Wistar大鼠随机分为3组:正常组、模型组和针刺组,每组12只。除正常组外,其余各组均接受18 d不同的应激。应激18 d后,再进行72 h快眼动睡眠剥夺造模。针刺组每天在应激前1 h进行针刺,连续21 d。21 d后,计算行为得分,取大鼠海马检测5-HT1A受体、5-HT2A受体mRNA水平。结果模型组大鼠自主活动较正常组明显减少(P<0.01);针刺组大鼠的自主活动较模型组明显增加(P<0.01)。与正常组相比,模型组大鼠海马5-HT1A受体mRNA表达明显下降,5-HT2A受体mRNA表达明显上升(均P<0.01);与模型组相比,针刺组大鼠海马5-HT1A受体mRNA表达上升、5-HT2A受体mRNA表达下降(P<0.05)。结论针刺可能通过调整5-HT受体间不平衡而发挥治疗SDD效应。

Objective To explore the molecular mechanism of acupuncture on sleep deprivation depression（SDD）.Methods 36 Wistar rats with similar Open-Field score were divided into normal group,model group,and acupuncture group,12 rats in each group.Except nor-mal group,the other groups received different stress for 18 d,and then were deprived rapid eye sleep for 72 h.Acupuncture group received acupuncture 1 h/d before the stress for 21 d.Their behaviors were observed and the levels of 5-hydroxytryptamine 1A and 2A receptors（5-HT1AR and 5-HT2AR） mRNA in hippocampus were determined.Results There was significant decrease in the autonomic-behavior in the model group compared with the normal group（P〈0.01）.The autonomic-behavior in acupuncture group increased compared with the model group（P〈0.01）.The expression of 5-HT1AR mRNA in hippocampus of model group decreased and the 5-HT2AR mRNA increased compared with the normal group（P〈0.01）.The expression of 5-HT1AR mRNA increased and the 5-HT2AR mRNA decreased in the hippocampus of acu-puncture group compared with model group（P〈0.05）.Conclusion Acupuncture is effective on SDD by re-balance the function of 5-HT re-ceptors.