绝经后妇女基质金属蛋白酶3和抑制因子水平及其与骨密度和骨桥蛋白的关系

Relationship between the levels of circulating matrix metalloproteinase-3 and its inhibitor and bone mineral density and osteopontin level in postmenopausal women

目的探讨绝经后妇女血清基质金属蛋白酶-3(MMP-3)和抑制因子(TIMP-1)水平及其与骨密度和骨桥蛋白的关系。方法将102名绝经后妇女用酶联免疫吸附试验(ELISA)测定的血清MMP-3、TIMP-1和骨桥蛋白(OPN)水平,计算MMP-3/TIMP-1比值,用双能X线吸收法(DEXA)测定腰椎正位,股骨颈,Ward's区和大粗隆的骨密度(BMD)。结果①骨质疏松组中MMP-3的数值(154±111)ng/ml高于正常组(124±103)ng/ml,而TIMP-1的数值(134±116)μg/L低于正常组(146±130)μg/L,血清OPN数值(57±19)ng/ml明显高于正常组(26±10)ng/ml。②骨质疏松组中MMP-3与MMP-3/TIMP-1比值和血清OPN存在明显正相关性(P<0.05),MMP-3/TIMP-1比值与骨密度存在明显负相关性(P<0.05),TIMP-1和Ward's区骨密度存在明显正相关性(P<0.05),和血清OPN存在明显负相关性(P<0.05),校正年龄和体重指数后,以上数据相关性增高(P<0.05)。结论绝经后骨质疏松症妇女MMP-3/TIMP-1比值与骨密度和OPN具有关联性,MMP-3/TIMP-1比值升高可能为绝经后骨质疏松症伴随骨代谢转换过程增快的表现。

Objective To study the relationship between the serum levels of matrix metalloproteinasc-3 （MMP-3） and tissue inhibitor of matrix metalloproteinase-1 （TIMP-1） and the level of osteopontin and bone mineral density （BMD） in postmenopausal women. Methods The serum levels of MMP-3,TIMP-1, and OPN in 102 postmenopausal women were measured using ELISA. The ratio of MMP-3 to TIMP-1 was calculated. BMDs of the lumbar vertebrae, the femoral neck, and the Ward＇s region were measured using dual-energy X-ray absorptiometry. Results 1 ） Serum MMP-3 concentration was significantly higher in osteoporosis group （ 154± 111 ng/ml） than in age-matched normal controls （ 124± 103 ng/ml）. Serum TIMP-1 concentration was lower in osteoporosis group （ 134 ± 116 μg/L） than in age-matched normal controls （146 ±130μg/L）. Serum OPN was higher in osteoporosis group （57 ± 19 ng/ml） than in control group （26± 10 ng/ml）. 2） In osteoporosis group, the ratio of MMP-3/TIMP-1 was positively correlated with serum OPN （ P 〈 0.05 ） , but negatively correlated with BMD. TIMP-1 was positively correlated with BMD of Ward＇s triangle （ P 〈 0.05 ）, but negatively correlated with OPN （ P 〈0. 05 ）. After adjudging by age and BMI, the above correlations increased. Conclusion The ratio of MMP-3/TIMP-1 was correlated with BMD and OPN in postmenopausal osteoporotic women. The increase of MMP-3/TIMP-1 ratio may present the increased bone turnover state in postmenopausal osteoporosis.