IL-2基因工程菌生长密度对rhIL-2表达的影响

Influence of Growth Density of IL-2 Recombinant Bacterial Strain on rhIL-2 Expression

目的观察不同培养时间和细菌密度对重组人白细胞介素-2（rhIL-2）基因工程菌的收获量和rhIL-2表达量的影响。方法：用40L发酵罐进行了3批发酵试验。结果：工程菌于30℃培养4．5～5h，细菌生长密度（OD600）在1．7～2．0之间。在此条件下，工程菌再经42℃诱导培养4h，菌体的收获量和表达水平都比较理想，其湿重和表达量的均值分别可达6．3g／L（菌液）和31．6％。结论：诱导前不同培养时间和细菌密度能显著影响rhIL-2基因工程菌的产量和rhIL-2的表达率。

Objective To observe the influence of the different culture time and the density ofbacteria on the yield of the recombinant human interleukin-2 (rhIL-2) recombinant bacterial strain andthe rhIL-2 expression. Methods: Three lotS of fermentation tests were conducted in a 40 L fermentor.Results: After the interleukin-2 (IL-2) recombinant bacterial strain was cultured at 30℃ for 4.5 ~ 5hours, the growth density (OD600 ) of bacteria was 1 .7 ~ 2.0. As the recombinant bacterial strain wasfurther induced and cultured at 42℃ for 4 hours, the yield of the wet weight of bacteria and the rhIL-2expression would be more saisfactory. And the average wet weight of the bacteria and the rhIL-2 expres-sion were 6. 3 g/L (fermented liquid) and 31. 6 %, respectively. Conclusion: Before the induction, boththe different culture time and the bacterial density can significantly influence the yield of rhIL-2 recombi-nant bacterial strain and the rhIL-2 expression.