四川省脊髓灰质炎Ⅰ型野毒株的分子病毒学检测

Molecular Analysis of the Wild Type I Poliovirus Isolated in Sichuan Province.

１９９１年安岳县和１９９２年会东县各１例临床诊断为脊髓灰质炎（脊灰）的患者，从其粪便标本中各分离出１株病毒，血清学定型均为脊灰Ⅰ型病毒，经ＰＣＲ－ＲＦＬＰ（Ｐｏｌｙｍｅｒａｓｅ Ｃｈａｉｎ Ｒｅａｃｔｉｏｎ－ＲｅｓｔｒｉｃｔｉｏｎＦｒａｇｍｅｎｔＬｅｎｇｔｈＰｏｌｙｍｏｒｐｈｉｓｍ ａｓｓａｙ）法检测，２株病毒与Ｓａｂｉｎ Ⅰ有明显差异定为Ⅰ型野毒株。又经核酸序列测定，其与Ｓａｂｉｎ Ⅰ型疫苗株亦有明显的差异，证实为脊灰互型野毒株。结果显示，不同年份、不同地方流行株间的序列有差异。四川省脊灰流行与毒株间核酸序列的差异有关，但我国现行疫苗ＯＰＶ（ＯｒａｌＰｏｌｉｏＶａｃｃｉｎｅ）能有效预防我省脊灰，基础兔疫和强化免疫是预防和消灭脊灰的有力武器。

Two polio cases were clinically confirmed in 1991 and 1992, one was in Anyue county and another inHuidong county. Two strains of type I poliovirus were isolated form the stool sapliles. By PCR-RFLP the two isolateswere confirmed as wild poliovirus. The VPI apuence of 300 nucleotides of Sichuan inolates were analysed and comparedwith Sabin I vaccine strain. The homogeneous rate was 78% and 74% respectively, so they were confirmed as wildtype I poliovirus. The results showed that there were differences in sequence of epidemic strains in different places andtimes.