缺血再灌注时维生素E乳剂抗氧化作用的实验研究

Protecting Effect of Vitamin E on the Hepatic Peroxidization Injury by Ischemia and Reperfusion

目的 观察缺血再灌注时肝脏脂质过氧化损害及维生素Ｅ（ＶＥ） 乳剂的抗氧化保护效应。方法 采用大鼠全身低血压再灌注与肝缺血再灌注两种动物模型，检测肝脏组织丙二醛（ＭＤＡ）含量、超氧化物歧化酶（ＳＯＤ） 活性及三磷酸腺苷（ＡＴＰ）含量，同时进行肝组织病理形态学观察。结果 缺血再灌注可造成肝组织ＭＤＡ含量显著增加，ＳＯＤ活性及ＡＴＰ含量显著降低。给予ＶＥ 乳剂，可显著提高肝组织ＳＯＤ活性，减轻脂质过氧化反应损害。结论 脂质过氧化损害及能量代谢障碍是缺血再灌注所致组织细胞损伤的相关因素之一，ＶＥ

Objective To observe the protecting effect of vitamin E on liver peroxidization injury caused by ischemia and reperfusion. Methods By using two rat models of systemic and hepatic hypoperfusion and reperfusion, the MDA content, SOD activity and ATP contents in liver tissue were detected, and the pathohistological changes of the liver tissue were also observed. Results During hypoperfusion and reperfusion the liver tissue MDA content was significantly increased,SOD and ATP contents were significantly decreased compared with the placebo emulsion group ( P <0.01).In the V E emulsion group, the liver tissue SOD activity was obviously elevated, the lipid peroxidization injury was lessened, and the pathohistological changes of the liver tissue was mild in degree. Conclusions The free radicals induced in systemic and hepatic ischemia and reperfusion may be one of the related causes in the liver peroxidization injury,which may be improved by intravenous infusion of V E.