数字图像测定失代偿期乙型肝炎肝硬化肝组织纤维面积比例

Determination of the collagen proportionate area of hepatic tissue in HBV-related decompensated cirrhosis using digital image analysis

目的为了了解失代偿期肝硬化肝组织纤维面积比例,本研究采用数字图像分析定量测定因失代偿期乙型肝炎肝硬化行肝移植手术切除的病肝组织纤维化面积比例,以期了解失代偿期肝硬化肝组织纤维化程度。方法 53例因失代偿期慢性乙型肝炎肝硬化行肝移植手术切除的病肝组织,常规石蜡包埋、切片,并对肝组织标本行Masson染色,采用数字图像分析的方法,定量测定肝组织纤维化面积(CPA)。结果失代偿期肝硬化CPA均值为(35.93±14.42)%(11.24%～63.41%),同为失代偿期肝硬化,CPA可相差5倍以上。结论失代偿期肝硬化纤维化面积比例明显增加,CPA最高为63.41%。肝组织纤维化的程度与分布均是肝硬化的重要影响因素。

Objectives digital image analysis was used to quantitatively determine the collagen proportionate area（CPA） of resected hepatic tissue after liver transplantation due to chronic hepatitis B - induced decompensated cirrhosis. Methods A total of 53 hepatic tissue samples resected from liver transplant patients with chronic hepatitis B - induced decompensated cirrhosis were collected. Masson stains of the hepatic tissue were performed. Digital image analysis was used to quantitatively determine the collagen proportionate area of the hepatic tissue. Results The mean collagen proportionate area at the decompensated cirrhosis stage was （35.93±14.42） % （ 11.24% - 63.41% ）. However, the collagen proportionate area of hepatic fibrosis varied up to fivefold at decompensated cirrhotic stage. Conclusion The collagen proportionate area at the decompensated cirrhosis stage was significantly increased, the maximum collagen proportionate area was 63.41%. The density and distribution of fibrosis are also important factors of cirrhosis.