咖啡酸苯乙酯对实验性肝损伤大鼠的抗氧化作用

Antioxidative effect of caffeic acid phenethyl ester on chronic liver injury in rats

目的探讨蜂胶提取物咖啡酸苯乙酯(CAPE)对四氯化碳(CCl4)等复合因素诱导的肝损伤大鼠的抗氧化作用。方法取95只健康雄性SD大鼠,随机分为9组。A:正常对照组;B:溶剂对照组,皮下注射橄榄油、腹腔注射10%乙醇,纯水灌胃;C:单纯模型组,腹腔注射10%乙醇;D:维生素E组,10 mg/kg,腹腔注射,1次/d;E～I:CAPE(10%乙醇溶液)干预组:腹腔注射,3 mg/kg、6 mg/kg和12 mg/kg;灌胃,12 mg/kg和24 mg/kg,1次/d。C～I组均予以40%CCl4橄榄油溶液皮下注射、30%乙醇灌胃,高脂饲料作为单一饲料。同时每组给予对应药物处理10周。实验第10周处死大鼠。测定肝组织匀浆中氧化应激指标:丙二醛(MDA)、谷胱甘肽(GSH)水平以及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活力。肝组织标本行常规HE、Van Gieson染色。结果与单纯模型组的各项氧化应激指标相比较,腹腔注射CAPE 6、12 mg/kg两剂量组的肝组织中MDA含量明显降低、GSH水平升高、CAT和SOD活力增加(P<0.05)。腹腔注射CAPE 12 mg/kg组对肝脏氧化应激指标的改善程度比灌胃给药两个剂量组好(P<0.05)。腹腔注射CAPE 12 mg/kg组经HE和VG染色观察可见小部分区域肝细胞炎症坏死,少量纤维增生,较单纯模型组肝损伤程度轻。结论 CAPE可以抑制脂质过氧化,提高肝脏抗氧化能力。在本课题观察的剂量范围内,CAPE腹腔给药的抗氧化作用好于灌胃给药途径。

Objective To evaluate the antioxidative effect of caffeic acid phenethyl ester （CAPE） on the liver injury induced by carbon tetrachloride （ CCI4 ） et al. Methods Ninety - five Sprague - Dawley male rats were divided randomly into nine groups as follows ： A ： normal control, B：solvent control （injected olive oil via the s.c. route and 10% alcohol via the peritoneum, water was administered orally once a day）, C：model control（ 10% alcohol via the i.p. once a day）, D：VitE group （dissolved in olive oil; 10mg/kg, i.p. once a day） and E I：CAPE groups （dissolved in 10% alcohol; 3 mg/kg, 6 mg/kg, 12 mg/kg, via the i. p. ; and 12 mg/kg, 24 mg/kg, via the i.g. once a day）. C - I ： the groups were injected 40% CCl4 via the s. c. routes, fed with high - lipid forage and given 30% alcohol orally, except norreal control and solvent control. GSH and MDA levels, CAT and SOD activity of liver homogenate were quantified. Histological examination of the liver tissue sections used hematoxylineosin staining and Van Gieson staining. Results MDA level decreased while GSH levels, CAT and SOD activity increased in the CAPE treated groups, compared with model group（P 〈0. 05 ）. The improvement of the indexes was better in CAPE （6mg/kg, 12 mg/kg, via i.p. route） groups than that in model group （P 〈0.05）. Pathological examination showed hepatocyte necrosis and inflammation of minimal area and little fibrosis in CAPE （12 mg/kg, via i.p. route） group, which is less than that in model group. Conclusion CAPE could inhibit lipid peroxidation and enhance antioxidant capacity. Additionally, within the dose range, intraperitoneal injection was much more effective than orally administration.