代谢性损害对培养的海马神经元形态结构和Ca^(2+)含量的影响

Changes of morphology and [Ca（2+）] i in cultured hippocampal neuronal cells following metabolic insults

目的研究代谢性损害对培养的海马神经元形态结构和胞浆内Ｃａ2+含量的影响以及ＮＧＦ的保护作用。方法采用新生大鼠海马神经元培养技术，应用相差显微镜观察形态结构和用激光共聚焦扫描显微镜检测Ｃａ2+变化。结果去葡萄糖１２ｈ细胞表面有受损迹象，２４ｈ更为明显，两组细胞的存活率与其它组相比有显著差异。去葡萄糖６ｈ，细胞浆内Ｃａ2+就有升高的趋势，１２ｈ后，胞浆内Ｃａ2+浓度明显升高，荧光值是基础值的３－５倍。结论细胞内Ｃａ2+升高早于细胞形态受损时间，且出现钙振荡现象，这可能是细胞早期受损的一种表现，ＮＧＦ是通过维持细胞的钙稳态而对海马神经元起保护作用的。

Objective To explore change of of morphology and ([Ca2+] i) following metabolic insults incultured hippocampal neuronal cells. Methods The morphology was oberved with a phase-contrast inverted microscopy following the onset of glucose deprivation-induced neuronal damage in rat hipocampal all culture ,as well as the intrtaneuronal calcium levels ([Ca2+] i) by confocal laser scanning microscopy. Results After 12h of glucosedeprivation, the cell was damaged and the survival neuronal cell rate were significantly lower in 12h and 24h of glucose deprivation than that of the Other group. Direct measmements of intraneuronal calcium levels demonstrated sustained elevations of ([Ca2+] i ) after 6h and 12h of the glucose deprivation. Conclusions The elevation of[Ca2+] i) was earlier than that time of the damage of the cells, and the calcium oscillation may be a early damagesignal. NGF may serve ([Ca2+] i) stabilizing to Protect neural functions in the brain.