体外及裸鼠体内培养对甲状腺HLAⅡ类抗原阳性细胞数的影响

Influence of Culture in Atmosphere of Air, High-Oxygen , Balb/c Nude Mouse and Irradiation of Cobalt on the Number of HLAⅡ Positive Cells of Thyroid

目的:探讨空气、高氧、60钴照射后体外培养及裸鼠体内培养对甲状腺HLAⅡ类抗原阳性细胞数的影响。方法:采用免疫组织化学方法测定HLAⅡ类抗原阳性细胞数,并进行相互比较。结果:HLAⅡ类抗原阳性细胞数减少以60钴照射后裸鼠体内培养组最明显,空气、高氧、单纯60钴照射后短时间体外培养对HLAⅡ抗原阳性细胞数无显著影响。结论:经60钴照射后裸鼠体内培养对甲状腺HLAⅡ抗原阳性细胞数的减少作用较其它方法为佳,且具有操作简单、不易被污染等优点,值得推广应用。

Objective: To study the influence of culture in atmosphere of air, high-oxygen, Balb/c nude mouse and irradiation of cobalt on the number of HLAⅡ positive cells of thyroid. Methods It was calculating HLA Ⅱ positive cells of thyroid by immunohistochemistry and contrasting the results of the influence. Results There as no significant influence on HLA Ⅱof thyroid of culture in atmosphere of air, high-oxygen, and only after irradiation of cobalt, but culturing in Balb/c nude mouse after irradiation of cobalt decreases the HLA Ⅱpositive cells in thyroid markedly. Conclusion Culturing in Balb/c nude mouse after irradiation of cobalt is best method of decreasing the HLA Ⅱof thyroid. It is simple and uneasy to be contaminated.