人类胃粘膜上皮永生细胞株中APC、Rb、c-met基因拷贝数研究

Study of APC, Rb, c- met gene copy numbers of human gastric mucosa epithelial cell line GES 1

目的 在分子水平研究人正常胃粘膜上皮永生细胞株(GES1) 多种已知癌基因、抑癌基因的细胞内拷贝数,并对这一细胞的特性进行鉴定。方法 用载有APC、Rb 、cmet 基因的质粒经切口平移直接标记生物素制作探针,与GES1 间期细胞核原位杂交(FISH) 。结果 APC 基因双拷贝的核为48 % ,三拷贝的核占22 % ;Rb 基因双拷贝核占71 % ;cmet 基因双拷贝的核占80 % 。结论 GES1细胞系在分子水平介于正常与恶性之间。

Objective To Study the copy number of oncogene/tumor suppressor genes in GES 1 cell line to identify its characteritics.Methods Bio 14 dATP was incorporated into APC, Rb, c met gene cloned in plasmid by nick translation. Fluorescence in situ hybridization (FISH) of interphase nuclei of GES 1 cells was performed.Results Two copies of APC were shown in 48% interphase nuclei and 3 copies in 22%; 71% and 80% cells had normal copies of Rb and c met genes in their nuclei, respectively.Conclusion GES 1 cell line is a relatively normal gastric mucosa epithelial cell line and can be used as a human in vitro model system for the study of carcinogenesis.