FHIT基因与喉癌相关性的研究

FHIT gene is abnormal in laryngeal carcinoma

目的 研究FHIT基因在喉癌发生中的作用。方法 应用RTPCR 技术筛查了喉癌细胞系Hep2 、宫颈癌细胞系HeLa 和20 例原发性喉癌的FHIT 基因转录本,对其中的2 例异常转录本进行了测序分析。同时,对另外60 例原发性喉癌FHIT 基因进行了微卫星多态分析。结果 在20 例喉癌中,14 例(70-0 % ) 显示FHIT 基因异常转录本,Hep2 和HeLa 细胞系FHIT基因转录本均存在缺失。测序发现,异常的FHIT基因转录本常缺失多个外显子,而且多发生于第5 和( 或) 第8 外显子。微卫星多态分析表明,FHIT基因在喉癌中既存在杂合性丢失,又存在微卫星不稳定现象,但以前者为主。结论 FHIT 基因参与了喉癌的发生,可能是喉癌候选抑癌基因之一。

Objective To determine the role of FHIT gene in laryngeal carcinogenesis. Methods Laryngeal cancer cell line Hep 2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT PCR, sequencing and microsatellite analysis. Results Abnormal transcripts of FHIT gene were found in the two cell lines and 70%(14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both loss of heterozygosity and microsatellite instability of FHIT gene existed in laryngeal carcinoma and the former was more common. Conclusion FHIT gene participates in the laryngeal carcinogenesis and may be one of the candidate tumor suppressor genes.