谷氨酸受体在延髓网状背侧亚核内的分布

DISTRIBUTION OF GLUTAMATE RECEPTOR IN THE SUBNUCLEUS RETICULARIS DORSALIS OF THE RAT

谷氨酸是哺乳类中枢神经系统的一种重要的神经递质。为了探讨谷氨酸是否作用于延髓网状背核的神经元 ,采用免疫组织化学方法 ,对代谢型谷氨酸受体的 7种亚型 ( m Glu R1-7)以及离子型谷氨酸受体中的 N-甲基 -天门冬氨酸 1型受体 ( NM-DAR1)在延髓网状背侧亚核内分布的情况进行了研究。结果显示 ,几种谷氨酸受体的免疫阳性产物主要定位在神经元的胞体和树突上。在延髓网状背侧亚核可以观察到 m Glu R5、m Glu R7和 NMDAR1这 3种受体亚型的免疫阳性神经元 ,其中 m Glu R7免疫阳性结构主要为终末样结构 ,而 NMDAR1免疫阳性结构主要定位于神经元胞体。在该核内未见 m Glu R2 -3、m Glu R4以及 m Glu R6等受体亚型的免疫阳性神经元。研究结果表明 ,延髓网状背侧亚核是谷氨酸的作用部位 。

Glutamate is an important neurotransmitters in the mammalian central nervous system. In an attempt to reveal the functional site of glutamate in the subnucleus reticularis dorsalis (SRD), the distribution of metabotropic glutamate receptor subunits (mGluR1～ mGluR7) and N-methyl-D-aspartat receptor subunit (NMDAR1) was immunocytochemically investigated in adult rat. We found that several GluR-Like immunoreactivity (LI) was mostly localized at the neuronal cell bodies and dendrites. The mGluR5-, mGluR7- and NMDAR1 like immunoreactive (LI) neuronal cell bodies, dendrites and axons were observed in SRD. Among those three type receptors, NMDAR-LI neuronal cell body and mGluR7-LI terminals were the most numerous. No mGluR2-3-, mGluR4 and mGluR6-LI were observed in SRD. These findings confirmed that subneucleus reticularis dorsalis is sites of the glutamate. It suggested that the glutamate is significantly related to the regulation of the SRD neurons.