原发性肝癌组织中ALP及其同工酶的临床研究

THE STUDY OF ALKALINE PHOSPHATASE AND ITS ISOENZYMES IN HEPATIC TISSUES OF PATIENTS WITH PRIMARY HEPATIC CARCINOMA

目的 :研究原发性肝癌 ( PHC)患者癌组织碱性磷酸酶 ( ALP)及其同工酶的变化特征及机理。方法 :检测 PHC病人肝癌组织及癌旁组织中 AL P比活性及其同工酶 ,并对其结果进行分析。结果 :2 7例 PHC肝癌组织标本中 ,3例检测到 AL P 区带 ,阳性率为 11.1% ,而癌旁组织中均未有 AL P 的检出 ;ALP 在癌旁组织中检出率达 10 0 % ,而肿瘤组织仅 14 .8% ( P<0 .0 1) ,且均在两种组织的可溶性 AL P中检出。 AL P总活性表现为癌旁组织高于肿瘤组织 ,肿瘤组织以可溶性 ALP为主。结论 :AL P 为肝癌组织所合成 ,为肝癌特异性同工酶。癌旁组织中 ALP 高表达 ,ALP 不能作为

Objectives: To investigate the characteristics of alkaline phosphatase (ALP) and its isoenzymes in hepatoma tissues of patients with primary hepatic carcinoma (PHC). Methods: The ALPs and its isoenzymes in liver tissues of patients with PHC were investigated by biochemical analysis. Results: Among 27 cases with PHC, ALPⅠwas positive in 3 cases hepatoma tissues, with a positive rate of 11.1%. No ALP Ⅰ was found in liver tissues adjacent to hepatoma. ALP Ⅴ was detected in all liver tissues adjacent to hepatoma, while it was positive in only 14.8% hepatoma tissues. ALPⅤ was present as soluble ALP form. The total ALP activities was higher in the tissues adjacent to hepatoma than hepatoma tissues. ALP in hepatoma tissues was mainly in soluble form. Conclusions: ALP Ⅰ is synthesized by hepatoma tissues and it is a hepatoma-specific isoenzyme. ALP Ⅴ is overexpressed in liver tissues adjacent to hepatoma, so ALP Ⅴcan't be regarded as a tumor marker.