人肝癌及癌周组织中总RNA和NOS的表达与改变

EXPRESSION AND ALTERATIONS OF TOTAL RNA LEVELS AND NOS ACTIVITIES IN HUMAN HEPATOMA AND ITS PARACANCEROUS TISSUES

目的 :探讨一氧化氮合酶 ( NOS)在人肝癌发生过程中的表达及作用机制。方法 :以病理组织学和分子生物学方法 ,分析16例自身配对的肝癌、癌旁组织和远癌组织中总 RNA浓度 ,并以重氮法和硝酸还原酶法分别定量不同组织中 NOS的比活性和一氧化氮 ( NO)浓度。结果 :经病理组织学证实的 16例肝癌、癌旁和远癌组织中 :1从癌灶→癌旁→远癌组织中 ,总 RNA浓度呈逐步升高趋势 ,三种不同组织间的差异均有显著性 ( P<0 .0 5 ) ;2癌灶、癌旁、远癌三种组织中 NOS比活性持续上升 ;( 3 )肝癌组织中 NOS比活性改变与肝总 RNA浓度、或与 NO水平呈明显正相关 ,其相关系数分别为 γ=0 .5 8( P<0 .0 1)和 γ=0 .47( P<0 .0 5 )。结论 :资料提示 NOS活性的异常改变 。

Objective: To explore the expression and act mechanism of nitric oxide synthase (NOS) during development of human hepatocellular carcinoma (HCC). Methods: The toatl RNA concentration, the specific activities of NOS and nitric oxide (NO) level in 16 self-paired HCC, its adjacent paracancerous and distal cancerous tissues were investigated by pathological examination and molecular biological assay. Results: Of the 16 liver tissues confirmed by pathological examination, (1) An increasing tendency of total RNA concentrations and significant difference (P<0.05) was found from cancer to distal cancerous tissues; (2) The specific activities of NOS was increased gradually from hepatoma to its distal cancerous tissues;(3)The specific activities of NOS in hepatoma tissues were positively correlated with the NO levels (γ=0.47, P<0.05) or the hepatic total RNA concentrations (γ=0.58, P<0.01). Conclusion: The present data suggest that abnormal expression of liver NOS might play aprotecting role in development of hepatocyte canceration.