异体骨髓间充质干细胞移植提高皮肤扩张效率

Transplantation of ailogeneic mesenchymal stem cells into skin expansion

目的探讨异体骨髓间充质干细胞（bone marrow—derived mesenchymal stem cells，BM—MSCs）在皮肤扩张中发挥的作用，为临床更好地使用皮肤扩张术提供新的方法。方法体外分离、培养乳猪BM—MSCs。小型猪随机分为A、B、c、D4组，前3组脊柱两侧各埋置3个扩张器，A组扩张局部注射BM—MSCs、B组耳静脉注射BM—MSCs、C组仅埋置扩张器、D组为对照组。定期注水，测量各组扩张第7、14、28天标记面积，免疫荧光检测BM—MSCs的分化作用。结果流式鉴定BM—MSCs示CD90、CD29阳性表达，CD34、CD45阴性表达。扩张第28天，A组面积较c组增加明显（P〈0．01），B组与C组之间比较差异具有统计学意义（P〈0．05）。免疫荧光示CD31、PCNA阳性表达率A、B组大于C、D组。结论异体移植的BM—MSCs能有效促进扩张皮肤新生，以局部移植的效果明显。

Objective To investigate the feasibility of transplantation of mesenchymal stem cell （aisc） into expanion skin. Methods MSCs were isolated from porker＇s bone marrow and cultured in vitro. Pigs were randomly divided into four groups： Group A was injected with MSCs on the local expansion; Group B was injected with MSCs from ear vein; Group C was planted expander only; Group D was the contronl group. Each side of pig＇s spinal column was implanted with three expander in groups A, B and C. The same volume of NS was injected at the fixed time, the marked area measured after 7, 14, 28 days of expansion, the difference of those area was compared between groups. The differentiation of BM-MSC was detected by immunofluorescence. Results Flow-cytometric analysis showed that these 13MSCs expressed CD90 and CD29 highly but did not express CD34 or CD45. The expansion area （cm2） of groups A, B, C and D was 34.05±0.92, 31.83±1.07,30. 10±0.79, and 18.27±0.25, respectively （P〈0.01）. Immunofluorescenee showed that the positive expression rate of CD31, PCNA in groups A and 13 was higher than that in groups C and D, in which the expression was the highest in group A. Conclusions Allogeneic transplantation of BM-MSC can promote skin expansion and the effect of local transplantation group is most significant.