毛细管电泳测定钩藤中钩藤碱、异钩藤碱、去氢钩藤碱的含量

Determination of rhynchophyline,isorhynchophyline and dehydrogenase rhynchophyline in uncaria by HPCE

建立了钩藤中钩藤碱、异钩藤碱、去氢钩藤碱的含量的测定方法：采用高效毛细管电泳法，运行缓冲液为25mmol／L磷酸盐缓冲液（磷酸调pH为2．5）-甲醇（体积比为4：1），运行电压21kV，柱温25℃，检测波长250nm。结果：钩藤碱、异钩藤碱、去氢钩藤、线性范围分别为0．00176～0．01760mg／mL（r=0．9998），0．00183～0．01830mg／mL（r=0．9996），0．00101—0．01010mg／mL（r=0．9998），加样回收率（n=6）分别为99．73％（RSD1．17％）、99．23％（RSD1．23％）、98．91％（RSD1．02％）。试验证明该法操作简便，分析快速，结果准确，重现性好，适用于立钩藤中钩藤碱、异钩藤碱、去氢钩藤碱的含量的测定。

Objective： To establish a new method for determination of rhynchophyline, isorhynchophyline and dehydrogenase rhynchophyline in Uncaria. Method：rhynehophyline,isorhynchophyline and dehydrogenase rhynchophyline in Uncaria were determined by using high performance capillary electrophoresis （HPCE）, The background electrolyte system selected was 25 nmol-L-1 phosphate buffe（ pH = 2. 5 ）-methanol（4：1 ）. applied voltage was 21 kV ;temperature was 25℃. diode array detector set at 250 nm. Results： the linear range of rhynchophyline, isorhynchophyline and dehydrogenase rhynchophyline was 0. 00176 - 0. 0176 mg · mL^-1 （ r = 0. 9998 ）, 0. 00183 - 0. 0183 mg · mL^-1 （ r = 0. 9996） ,0. 00101 -0. 0101 mg·mL^-1 （r =0. 9998） ,The average recovery was99. 73% （ RSD1.17% ） ,99.23% （ RSD 1.23% ） and 98.91% （RSD 1.02% ）. Conclusion：The method is simple,rapid and reproducible,and could be used for determination of rhynchophyline,isorhynchophyline and dehydrogenase rhynchophyline in Uncaria.