代森锰锌对小鼠生精细胞T型Ca^(2+)通道的作用

Effects of mancozeb on T-type calcium channels in mouse spermatogenic cells

目的:研究农药代森锰锌(mancozeb)对小鼠生精细胞T型钙电流(ICaT)的影响。方法:采用膜片钳全细胞记录技术观察代森锰锌对急性机械分离的小鼠生精细胞ICaT的影响。结果:Mancozeb(10、20、40、80、120μmol/L)呈浓度、电压依赖性抑制小鼠生精细胞钙电流,抑制率分别为(9.93±0.90)%、(20.93±2.50)%、(25.92±2.80)%、(42.64±3.10)%、(56.80±3.20)%(n=6,P<0.05)。Mancozeb对T型钙通道的半数最大抑制浓度(K50)为35.60μmol/L。120μmol/L mancozeb显著改变T型Ca2+通道的激活和失活特性:半数激活电压(V1/2a)和激活斜率因子(κa)分别从(-47.09±1.05)mV和(8.67±0.78)mV变为(-51.46±0.84)mV和(10.56±0.619)mV(n=5,P<0.05);而半数失活电压(V1/2i)和失活斜率因子(κi)分别从(-62.96±3.36)mV和(9.93±1.41)mV变为(-64.11±5.55)mV和(13.64±1.95)mV(n=5,P<0.05)。结论:Mancozeb对生精细胞T型钙通道有抑制作用,且呈浓度依赖性。

Objective：To investigate the effect of mancozeb on T-type calcium currents（ICaT） in spermatogenic cells.Methods：Ca2＋ currents were obtained in acutely dissociated mouse spermatogenic cells by the whole-cell patch clamp technique and the effects of mancozeb on ICaT were observed.Results：Mancozeb at the concentrations of 10,20,40,80 and 120 μmol/L significantly inhibited ICaT in the mouse spermatogenic cells,with the K50 value of 35.60 μmol/L and the inhibition rates of mancozeb were（9.93±0.90）%,（20.93±2.50）%,（25.92±2.80）%,（42.64±3.10）% and（56.80±3.20）%,respectively（n=6,P 0.05）.Mancozeb of 120 μmol/L significantly changed the activation and inactivation of ICaT：the half activation potential（V1/2a） and the activation steepness factor（κa） from（-47.09±1.05）mV and（8.67±0.78）mV to（-51.46±0.84）mV and（10.56±0.619）mV,respectively（n=5,P 0.05）;the half inactivation potential（V1/2i） and the inactivation steepness factor（κi） from（-62.96±3.36）mV and（9.93±1.41）mV to（-64.11±5.55）mV and（13.64±1.95）mV,respectively（n=5,P 0.05）.Conclusion：Mancozeb has significant inhibitory effects on ICaT in mouse spermatogenic cells with concentration dependence.