胃癌患者血清IL-6、CRP水平的变化及其与肿瘤血管生成的关系

The changes of serum levels of IL-6 and CRP in patients with gastric carcinoma and the relationship with angiogenesis in neoplasms

目的:观察胃癌患者血清白细胞介素-6(IL-6)、C-反应蛋白(CRP)的水平变化,同时研究胃癌组织中血管内皮生长因子(VEGF)、CD31的表达及其与血清IL-6水平的关系。方法:应用酶联免疫吸附试验(ELISA)测定IL-6的水平,采用Beckman蛋白测定系统检测CRP,应用免疫组化技术检测VEGF、CD31在胃癌中的表达。结果:胃癌患者血清IL-6水平较对照组明显升高(P<0.001),并与肿瘤病理分期有关,肿瘤切除后血清IL-6水平明显降低(P<0.001);VEGF的阳性细胞百分数和CD31抗体标记的微血管密度(MVD)均随病理分期增高而升高,且均与血清IL-6水平呈正相关。结论:血清IL-6、VEGF、CD31的检测有助于胃癌的早期诊断与治疗,并提示病情及预后。

Objective：To study the changes of serum levels of interleukin-6（IL-6） and C-reactive protein（CRP） in patients with gastric carcinoma and the relationship of the expression of vascular endothelial growth factor（VEGF） and CD31 with the serum level of IL-6.Methods：IL-6 was evaluated by enzyme-linked immunosorbent test（ELISA）.CRP was detected by Beckman albumen measuring system.The expression of VEGF and CD31 in gastric carcinoma tissues was detected by immunohistochemistry.Results：The serum level of IL-6 in patients with gastric carcinoma was significantly higher than controls（P 0.001） and concerned with staging.After operation,the serum level of IL-6 decreased significantly（P 0.001）.The percentage of VEGF positive cells and the microvessel density（MVD） labeled by CD31 antibody gradually increased with the staging.They were all positively correlated with the serum level of IL-6.Conclusion：The detection of serum IL-6、VEGF and CD31 in gastric carcinoma patients is useful to early diagnosis,therapy and to anticipate the condition and prognosis.