JNK抑制剂及NAC对氧糖剥离后复氧复糖所致脊髓神经元损伤的保护作用

The study of JNK inhibitor and NAC on oxygen-glucose deprivation/regain induced neuronal apoptosis in cultured spinal neuronal cells

目的:初步探讨JNK(The c-Jun NH-terminal kinase)抑制剂及N-乙酰半胱氨酸(N-acetylcysteine,NAC)对脊髓神经细胞氧糖剥夺复糖复氧的保护作用。方法:离体脊髓神经元氧糖剥夺复糖复氧损伤(oxggen-glucose deprivation/regain,OGD/R)模型建立。随机分组:A组对照组(n=6);B组JNK抑制剂组(n=6);C组NAC处理组(n=6)。分别于脊髓神经元氧糖剥夺(oxygen-glucose deprivation,OGD)4 h后,再复氧复糖4、12、24 h,应用MTT法检测细胞凋亡。结果:成功建立OGD/R模型;脊髓神经元OGD/R后,B组和C组MTT各时间点吸光值均高于A组,但B组和C组间差异不显著。结论:JNK抑制剂及NAC能有效抑制体外培养的脊髓神经元氧糖剥夺复糖复氧所引起的凋亡。

Objective：To study the protective effects of JNK（the c-Jun NH-terminal kinase）inhibitor and NAC（N-acetylcysteine） on neuronal apoptosis induced by oxyen-glucose deprivation / regain in vitro.Methods：The oxygen-glucose deprivation / regain（OGD / R） models were established in vitro.The neurons were exposed to OGD 4h before being placed into normal culture medium.Group A was taken as a blank control.Spinal cord neurons received the JNK inhibitor and NAC treatment before regaining the oxygen-glucose in group B and C,respectively.The neuronal apoptosis were detected by MTT assay in different time points（4 h,12 h,24 h after regaining oxygen-glucose）.Results：The model of OGD / R in spinal cord neurons was successfully established.Cell viability in MTT assay were higher in group B and group C than that in group A,and there was no significant difference between group B and group C.Conclusion：The JNK inhibitor and NAC can effectively protect neurons from apoptosis induced by OGD / R.