CpG寡核苷酸促进Toll样受体9的表达增强乳腺癌细胞侵袭

CpG oligonucleotides activating Toll-Like receptor 9 promotes invasion of breast cancer cells

目的:探讨Toll样受体9(Toll-like receptor 9,TLR9)在乳腺癌细胞中的表达对其侵袭能力的影响。方法:应用West-ern blot检测乳腺癌细胞系TLR9的表达状况,以CpG寡核苷酸刺激不同乳腺癌细胞株后,检测TLR9表达的变化、体外侵袭能力的变化以及细胞活性的影响。结果:人乳腺癌细胞株MDA-MB-231表达TLR9而MCF-7不表达。CpG寡核苷酸处理后MDA-MB-231的TLR9表达增高,且侵袭能力进一步增强;MCF-7的该受体表达情况和侵袭能力均无明显变化。侵袭能力的增加不是源自于细胞活性的增强。结论:TLR9在高侵袭的乳腺癌细胞系中表达,其表达的增加可以促进乳腺癌细胞的侵袭能力。

Objective：To investigate the relationship between Toll-like receptor 9（TLR9）expression of breast cancer cells and their invasion.Methods：After using CpG oligonucleotides to stimulate breast cancer cells,the expression level of TLR9,the viability and invasion of breast cancer cells were examined by Western blot,Transwell and MTT.Results：TLR9 was expressed in MDA-MB-231 and could be promoted by CpG oligonucleotides,but was not in MCF-7.The invasion was enhanced while TLR9 up-regulated.Conclusion：TLR9 plays an important role in the invasion process of breast cancer.