$$$$超高效液相色谱法同时测定碳酸饮料中的22种食品添加剂

Simultaneous Determination of 22 Food Additives in Carbonated Beverages by Ultra High Performance Liquid Chromatography

建立了超高效液相色谱(UPLC)同时测定碳酸饮料中22种食品添加剂的方法。采用Waters BEH C18(1.7μm,2.1 mm×50 mm)色谱柱,以0.01 mol/L乙酸铵(pH 5.8～6.0)和乙腈为流动相进行梯度洗脱,在35℃柱温,0.2 mL/min流速下,采用二极管阵列检测器在217、232、257 nm波长处进行检测,外标法定量,15 min内可实现22种食品添加剂的分离。该方法具有良好线性,相关系数均大于0.995,检出限为0.02～0.50 mg/L,平均回收率为81%～107%,RSD值为1.0%～12.9%。该方法操作简单、灵敏度高、重复性好,可满足国内对碳酸饮料中添加剂的检测需求。

A simple and rapid method was developed for the simultaneous determination of 22 food additives in carbonated beverages by ultra high performance liquid chromatography （UPLC）. The sample was degassed by uhrasonie and added 10% ethanol. The pH was regulated to 8.0 using 25% ammonira - water （ 1 ： 1）. The separation was achieved on a BEH Cjs column（1.7 txm, 2. 1 mm x 50 ram） in 15 min by using 0.01 mol/L NH4Ae （pH 5.8 -6.0） and acetonitrile as mobile phase with a flow rate of 0.2 mL/min at 35 ~C. A diode array detector was used to detect at 217, 232 and 257 nm. The quantification was performed by the external standard method. The method showed good linearities for 22 food additives with correlation coefficients（r） more than 0. 995. The detection limits ranged from 0.02 mg/L to 0. 50 mg/L. The average recoveries for 22 food additives from spiked car- bonated beverage samples were in the range of 81% -107% with relative standard deviations（RSDs） of 1.0% -12.9%. With the advantages of simplicity, sensitivity and repeatability, the method could meet the requirements for determination of food additives in carbonated beverages at home.