不同产地中药赤芍在振荡体系中的化学指纹图谱研究

Study on Chemical Fingerprints of Traditional Chinese Medicine Radix Paeoniae Rubra in Oscillation System

采用H2SO4-CH2(COOH)2-Ce2(SO4)3-KBrO3化学振荡体系,研究了中药赤芍的化学指纹图谱,并对温度、赤芍用量进行了考察,确定体系的最佳实验条件为12 mL 3.0 mol/L H2SO4溶液、6 mL 0.4 mol/LCH2(COOH)2溶液、3 mL 0.005 mol/L Ce2(SO4)3和3 mL 0.2 mol/L KBrO3溶液,温度35℃、加入0.4 g的中药材粉末。通过对不同产地中药材赤芍(安徽亳州、山东菏泽、内蒙古赤峰、海拉尔)化学指纹图谱的研究,发现不同产地的中药材赤芍不仅具有明显不同的化学指纹图谱形状,而且主要参数也有很大区别。其中亳州赤芍的最高诱导时间较小(133.76 s),但振荡寿命最长(554.89 s);而海拉尔赤芍的诱导时间最长(214.17 s),但振荡寿命最短(237.11 s)。该化学指纹图谱可方便地用于不同产地中药材赤芍的区别和鉴定。

Chemical fingerprints of Radix Paeoniae Rubra（RPR）from different places of origin were studied by chemical oscillation system of H2SO4-CH2（COOH）2-Ce2（SO4）3-KBrO3.Effects of various variables on the chemical oscillation system were studied.The optimal conditions were as follows：12 mL 3.0 mol/L sulfuric acid,6 mL 0.4 mol/L malonate,3 mL 0.005 mol/L cerium ammonium sulfate,3 mL 0.2 mol/L sodium bromate and 0.4 g RPR powder at 35 ℃.The results indicated that,under the optimal conditions,the chemical fingerprints of RPR from different places of origin（Bozhou,Heze,Chifeng and Hailaer） showed significantly different characteristics,such as the maximum potential,induction time and oscillation lifetime,etc.The results also indicated that the RPR from Bozhou had the shortest induction time（133.76 s）,but the longest oscillation lifetime（554.89 s）.The RPR from Hailaer had the longest induction time（214.17 s）,but the shortest oscillation lifetime（only 237.11 s）.The chemical fingerprints could be used to identify the origins of RPR.The method is simple,rapid and sensitive,and without need sample preparation.