Cr(Ⅵ)对细胞增殖周期和细胞内蛋白质及DNA含量的影响

Effects of six valent chromium on cell proliferation cycle and cellular contents of protein and DNA

目的 研究六价铬［Ｃｒ （ Ⅵ）］ 对细胞增殖周期和细胞内蛋白质及ＤＮＡ 含量的影响。方法 应用流式细胞技术研究Ｋ２Ｃｒ２Ｏ７ ［Ｃｒ （ Ⅵ）］ 对人胚肺细胞增殖周期和细胞内蛋白质及ＤＮＡ 含量的影响。结果 低剂量Ｋ２Ｃｒ２Ｏ７ （０ ～１－２５０μｍｏｌ／Ｌ） 可刺激细胞增殖， ２－５００μｍｏｌ／Ｌ 及以上剂量Ｋ２Ｃｒ２Ｏ７ 可以抑制细胞增殖， 细胞多数被阻断在Ｓ 期（ Ｐ＜０－０１） 。细胞内蛋白质及ＤＮＡ 含量也随Ｋ２Ｃｒ２Ｏ７ 浓度增加而有降低趋势， 在５－０００μｍｏｌ／Ｌ 组， 即有差异显著性（ Ｐ＜０－０５） 。结论 低剂量Ｋ２Ｃｒ２Ｏ７ （０ ～１－２５０μｍｏｌ／Ｌ） 对细胞增殖的刺激作用， 可能与六价铬多阶段致癌作用有关。

Objective To study the effects of six valent chromium[Cr(Ⅵ)]on cell proliferation cycle and cellular contents of protein and DNA.Methods Effects of potassium dichromate[Cr(Ⅵ)]on cell proliferation cycle and cellular contents of protein and DNA in human embryo lung(HEL)cells were detected by flow cytometry.Results Low dose potassium dichromate (0～1 250)μmol/L) could stimulate cell proliferation,and a dose of 2 500 μmol/L or more could inhibit it and most of cell cycles were delayed at S phase(P<0 01).Cellular contents of protein and DNA showed a reducing trend with increasing doses of potassium dichromate,and there was significant difference in them with treatment of 5 000 μmol/L potassium dichromate(P<0 05).Conclusion Stimulation of cell proliferation cycle by low dose of potassium dichromate could correlate to the multistage carcinogenicity of Cr(Ⅵ).