贝那普利对糖尿病大鼠胰腺组织中TGF-β_1和Smad3表达的影响

Effect of Benazepril on expression of transforming growth factor-β_1 and Smad3 in pancreatic tissue of Diabetic rats

目的研究贝那普利对糖尿病大鼠胰腺组织中TG F-β1和Sm ad3表达和R AS系统的影响。方法以健康成年W istar大鼠为实验对象,随机分为正常组、糖尿病未干预组和糖尿病贝那普利干预组3组。正常组以普通饲料喂养,糖尿病未干预组和糖尿病贝那普利干预组以高脂高糖饲料喂养。6周后腹腔注射链脲佐菌素诱导成糖尿病大鼠。于给药8周后处死。检测各组大鼠的血糖、胰岛素、体重。采用免疫组织化学方法检测胰腺组织中TG F-β1及Sm ad3蛋白的表达,并用彩色病理图像分析系统进行定量分析。采用放射免疫的方法检测胰腺组织中的血管紧张素Ⅱ的含量。结果与正常组相比,糖尿病未干预组血糖增加胰岛素分泌减少、体重减轻。TG F-β1及Sm ad3蛋白的表达明显增加。胰腺组织中的血管紧张素Ⅱ含量增加。贝那普利治疗后除体重无变化外上述指标均明显改善(P<0.05)。结论胰腺局部R AS系统和TG F-β1/Sm ad通路在糖尿病时是激活的,贝那普利治疗后可改善胰腺纤维化并可改善胰岛功能。

【Objective】 To investigate the effect of Benazepril on expression of TGF-β1 and Smad3 and RAS system in pancreatic tissue of diabetic rats.【Methods】 Healthy male Wistar rats were randomly divided into three groups： normal control group,diabetic rats group and Benazepril-treated group.The normal control group fed with normal diet,diabetic rats group and Benazepril-treated group fed with high fat and high sugar diet.After six weeks diabetic rats group and Benazepril-treated group were injected of streptozotocin to induced diabetic rats.Rats were sacrificed after treatment for eight weeks.Glucose,insulin,weight were observed.The sites and level of TGF-β1 and Smad3 protein were examined by immunohistochemistry and were quantitatively analyzed by color pathological image analysis system.Radioimmunoassay used to detect pancreatic tissue concentration of angiotensin Ⅱ.【Results】 Compared to normal control group,Glucose,insulin weight were significantly decreased in diabetic rats group.The level of TGF-β1 and Smad3 in pancreatic tissue were expressed significant increasingly.The angiotensinⅡwas increased in pancreatic tissue.Benazepril administration made all changes much better（P 0.05）.【Conclusion】 TGF-β1/ Smad and RAS system signaling is activated in diabetic nephrology.Benazepril treatment can improve the pancreatic fibrosis and islet function.