摘要
比较国产聚丙烯酰胺水凝胶（ＦＨ）与进口聚丙烯酰胺水凝胶（ＩＴ）的细胞毒性。用ＭＴＴ法检测Ｌ９２９ 细胞在不同浓度的两种聚丙烯酰胺中的相对增殖率（ＲＧＲ，％），按通用标准评价材料的细胞毒性。Ｌ９２９ 细胞在１２．５ ％ 的ＩＴ中２、４、６ 天时的ＲＧＲ分别为９６ ．６ 、９１．２ 和９０．９ ，在２５％ 的ＩＴ中为９３．１、８３ ．１ 和８９．３，在５０ ％ 的ＩＴ中为８９．７、８５．０ 和９０．９；而在１２ ．５ ％ 的ＦＨ中２、４、６ 天时的ＲＧＲ分别在９８．３、８４．４ 和９１．３ ，在２５ ％ 的ＦＨ中为９４．８、８６．９ 和８９．３ ，在５０ ％的ＦＨ中为８９．７ 、８７ ．５ 和９６ ．４；以上各组材料毒性级别均为“１”。ＦＨ 和ＩＴ都属于无细胞毒性的材料。
To compare the cytotoxicity of imported polyacrylamide hydrogel(IT) with that of home made(FH). The relative growth ratio (RGR, %) of L929 cells in IT or FH at different concentrations were tested with MTT assay, the cytotoxicity of the materials were evaluated by commonly used criteria. The RGR of L929 cells in 12.5% IT at day 2, 4 and 6 were 96.6, 91.2 and 90.9, in 25% IT were 93.1, 83.1 and 89.3, in 50% IT were 89.7, 85.0 and 90.9, respectively, that in 12.5% FH at day 2, 4 and 6 were 98.3, 84.4 and 91.3, in 25% FH were 94.8, 86.9 and 89.3, in 50% FH were 89.7, 87.5 and 96.4, respectively. The cytotoxicity of all the samples was graded “1” by the criteria. FH and IT were noncytotoxic materials.
出处
《实用美容整形外科杂志》
1999年第6期326-329,共4页
Journal of Practical Aesthetic and Plastic Surgery
