氢氧化铝吸附炭疽杆菌重组保护性抗原的影响因素

Influencing Factors of Aluminium Hydroxide-adsorbed Recombinant Protective Antigen of Bacillus anthraxcis

目的探讨氢氧化铝吸附炭疽杆菌重组保护性抗原(Recombinant protective antigen,rPA)的影响因素。方法取含不同PO43-浓度的PBS和添加EDTA的PBS,分别稀释rPA后,加入氢氧化铝,放置2～8℃及(23±2)℃作用不同时间,检测A280和A260值,计算未吸附的rPA蛋白量,并比较不同溶液中氢氧化铝对rPA的吸附效果。结果氢氧化铝对rPA的吸附率随PO43-浓度的增加而降低;PBS中添加EDTA后,氢氧化铝对rPA的吸附效果明显下降;2～8℃较(23±2)℃的吸附率略有增加,但不同时间的吸附量无明显差异;在H2O中氢氧化铝对rPA的吸附效率比在PBS中高。结论稀释缓冲液和离子强度、EDTA均可影响氢氧化铝与rPA的吸附效果,但吸附温度和时间对吸附的影响不大。

Objective To investigate the influencing factors of aluminium hydroxide-adsorbed recombinant protective antigen（rPA） of Bacillus anthraxcis.Methods The rPA was diluted with PBS containing various concentrations of PO43-and containing EDTA respectively,then added with aluminium hydroxide,incubated at 2 ～ 8℃ and（23 ± 2）℃ for various hours respectively and determined for A280 and A260,based on which the non-adsorbed rPA contents were calculated,and the adsorption effects of rPA with aluminium hydroxide in various solutions were compared.Results The adsorption rate of rPA with aluminium hydroxide decreased with the increasing concentration of PO43-.After EDTA was added into PBS,the adsorption rate decreased significantly.The adsorption rate at 2 ～ 8℃ was slightly higher than that at（23 ± 2）℃,while showed no significant differences at various time points.The adsorption efficacy of rPA with aluminium hydroxide in water was higher than that in PBS.Conclusion Dilution buffer,ion strength and EDTA influenced the adsorption of rPA with aluminium hydroxide,while the temperature and time for adsorption showed no significant influence.