Hedgehog信号通路阻断对人胃癌细胞SGC-7901侵袭和血管内皮生长因子表达的影响

Effect of Hedgehog Signal Pathway Blocking on Invasion and Vascular Endothelial Growth Factor Expression of Human Gastric Cancer SGC-7901 Cells

目的探讨Hedgehog(Hh)信号通路阻断对人胃癌细胞SGC-7901侵袭的影响及其可能的机制。方法免疫荧光检测Hh信号通路分子Shh和Gli1蛋白在SGC-7901细胞中的表达;用不同浓度(2.5、5、10μmol/L)的Hh信号通路特异性阻断剂环靶明作用SGC-7901细胞48 h后,Transwell小室试验检测SGC-7901细胞的侵袭能力,半定量RT-PCR检测SGC-7901细胞中Shh、Gli1和血管内皮生长因子(VEGF)基因mRNA的表达。结果 Shh和Gli1蛋白在SGC-7901细胞中高表达;环靶明能显著抑制SGC-7901细胞的侵袭,且呈剂量依赖性;Hh信号通路阻断后,SGC-7901细胞中Shh基因mRNA的表达水平无明显改变,Gli1和VEGF基因mRNA的表达水平显著下降。结论 Hh信号通路分子在SGC-7901细胞中高表达,阻断Hh信号通路可以抑制SGC-7901细胞的侵袭,可能是通过抑制Gli1下调VEGF起作用。

Objective To investigate the effect of Hedgehog（Hh） signal pathway blocking on invasion and vascular endothelial growth factor（VEGF） expression of human gastric cancer SGC-7901 cells as well as the relevant mechanism.Methods The expressions of Hh signal pathway molecules Shh and Gli1 proteins were determined by IFA.SGC-7901 cells were treated with cyclopamine,a specific blocker of Hh signal pathway,at various concentrations（2.5,5 and 10 μmol / L） for 48 h,then determined for invasion ability by Transwell test,and for expressions of Shh,Gli1 and VEGF mRNAs by semi-quantitative RT-PCR.Results Shh and Gli1 proteins were highly expressed in SGC-7901 cells.Cyclopamine showed a dosage-dependent inhibitory effect on the invasion of SGC-7901 cells.After Hh signal pathway blocking,the Shh mRNA level in SGC-7901 cells showed no significant change,while the Gli1 and VEGF mRNA levels decreased significantly.Conclusion Hh signal pathway molecules were highly expressed in SGC-7901 cells.Hh signal pathway blocking inhibited the invasion of SGC-7901 cells by a possible mechanism of down-regulating VEGF through inhibiting Gli1.