猪旋毛虫病胶体金检测试纸条的制备

Preparation of Colloidal Gold Test Strip for Swine Trichinellosis

目的制备猪旋毛虫病胶体金检测试纸条,并进行验证。方法采用免疫胶体金技术标记猪旋毛虫排泄-分泌(Excretory-secretory,ES)抗原,在AE98NC膜上点上兔抗猪IgG作为检测带,抗ES抗原抗体作为对照带,将NC印膜、胶体金抗原玻璃棉、吸水纸和支持板用胶膜组成试纸条,进行敏感性、特异性和重复性验证,并与ELISA检测方法进行对比。结果试纸条检测38头份猪旋毛虫阳性血清的敏感性为97.37%;检测33头份猪蛔虫阳性血清、26头份猪囊虫阳性血清、21头份猪细颈囊尾蚴阳性血清和98头份猪旋毛虫阴性血清的特异性为98.88%;重复性好;与ELISA检测方法的敏感性和特异性差异无统计学意义(P﹥0.05)。结论已制备猪旋毛虫病胶体金检测试纸条,该试纸条敏感、特异、简便、快速,可用于猪旋毛虫病的检疫及流行病学调查。

Objective To prepare and verify the colloidal gold test strip for swine trichinelosis.Methods The excretory-secretory（ES） antigen of swine trichina worm was labeled by immune colloidal gold technique and sprayed evenly onto glass wool.Rabbit anti-swine IgG was loaded onto AE98NC membrane as detection band,and anti-ES antibody as control band.NC printing membrane,glass wool loaded with colloidal gold-labeled ES antigen,water-absorbing paper and support plate were assembled into a test strip and verified for sensitivity,specificity and reproducibility,by which the detection result was compared with that by ELISA.Results Thirty-eight serum samples positive for swine trichina worm were detected by the prepared test strip,and the results showed a sensitivity of 97.37%.Thirty-three serum samples positive for swine ascarides,26 positive for pork tapeworm,21 positive for Cysticerus cellulose,and 98 negative for swine trichina worm were detected by the test strip,and the result showed a specificity of 98.88% and good reproducibility.No significant differences were observed in the sensitivity and specificity of the test strip and ELISA（P〉 0.05）.Conclusion A colloidal gold test strip for swine trichinelosis was prepared,which was sensitive,specific,simple and rapid and suitable for the quarantine and epidemiological investigation of swine trichinellosis.