低剂量辐射诱导免疫适应性反应的实验研究

Immunological adaptive response induced by low dose radiation

目的 研究低剂量辐射能否诱导免疫适应性应。方法 ３ＨＴｄＲ 掺入法检测淋巴细胞增殖率；ＣＴＬＬ依赖株检测ＩＬ２ 活性；单克隆抗体免疫荧光标记，流式细胞术（ＦＣＭ） 检测淋巴细胞亚组及膜受体表达；ＰＩ标记，ＦＣＭ 检测细胞周期及细胞凋亡；双抗体夹心间接ＥＬＩＳＡ 法测定可溶性白介素２ 受体；Ｆｕｒａ２ 负载，双波长荧光测定法检测细胞内［Ｃａ２＋ ］ｉ；传代培养细胞测定细胞倍增时间。结果 低剂量辐射预照射可明显减轻其后大剂量照射对诸多淋巴细胞功能的抑制作用。结论 低剂量Ｘ 射线可诱导多参数免疫适应性反应。

Objective\ To investigate whether immunological adaptive response could be induced by low dose radiation. Methods The following techniques were employed in this experiment:3H TdR incorporation for lymphocyte proliferation,a bioassay with CTLL cells for IL 2 activity,flow cytometry (FCM) for subsets and receptor expression of lymphocytes by their immunofluorescence stained with monoclonal antibodies,PI staining and FCM for cell cycle and apoptosis,sandwich ELISA for sIL 2R,fura 2AM loading for [Ca 2+ ]i,and a formula for doubling time of cells in culture. Results Suppressive effects of immune functions by challenge dose could be reduced by low dose pre irradiation. Conclusion Multiple immunological adaptive responses could be induced by low dose radiation.\;