罗非鱼维氏气单胞菌的分离鉴定和药敏试验

Isolation and Identification of Aeromonas veronii from Infected Tilapia Fry and Analysis of Its Drug Sensitivity

从患病罗非鱼(Oreochromis niloticus)鱼苗的病灶处分离到1株细菌GDZ1,人工感染试验显示,该分离株具有较强毒力。经ATB 32E细菌鉴定系统鉴定、补充生化鉴定和16SrRNA基因序列分析,确定该病原菌为维氏气单胞菌(Aeromonas veronii);药敏实验结果显示,29种药物中,米诺环素等15种化学试剂对该病原菌具有较强的抑菌作用。

A pathogenic bacterial strain GDZ1 was isolated from diseased tilapia fry cultured in a farm in Zhuhai,Guangdong.Artificial infection experiments showed that the isolated strain possessed strong virulence.Combining ATB 32E identification,supplementary biochemical identification and 16S rRNA sequence analysis, strain GDZ1 was identified as Aeromonas veronii. The drug sensitivity test of a total of 29 antimicrobial agents showed that the strain GDZ1 was highly sensitive to 15 agents such as minocycline,streptomycin,cefaclor and cefazolin.