bFGF对人牙髓、牙周膜成纤维细胞生物学效应的研究

Biological Effects of basic fibroblast growth factor on the cells of Pulp and Periodontium

目的:了解碱性成纤维细胞生长因子(bFGF)对人牙髓、牙周膜成纤维细胞的生物学效应,为bFGF在牙髓、牙周病中的治疗研究提供新的实验依据。方法:利用3H-TdR掺入法观察在bFGF作用下人牙髓、牙周膜成纤维细胞DNA和胶原蛋白合成的情况。结果:20ng/mL～60ng/mLbFGF可明显促进人牙髓、牙周膜成纤维细胞DNA的合成(P<0.01),在40ng/mL浓度时牙髓,牙周膜成纤维细胞DNA合成最高,40ng/mLbFGF作用于牙髓,牙周膜成纤维细胞,24～48h可使细胞DAN合成显著增多,牙髓成纤维细胞在36h时DNA合成达最高峰,牙周膜成纤维细胞在24h时DNA合成达最高峰;bFGF对牙髓,牙周膜成纤维细胞胶原蛋白的合成无明显促进作用(P>0.05)。结论:人牙髓、牙周膜成纤维细胞是bFGF的靶细胞,其胞膜上可能有bFGF特异性受体的存在,也表明bFGF在牙髓、牙周组织的创伤愈合中可能起重要作用。

Objective：To observe the biological effect of bFGF on fibroblasts from pulp and periodontal ligament and evaluate the value of bFGF in clinical pulpal and periodontal therapy.Method：DNA and collagen protein synthesis of human pulp and periodontal ligament fibroblasts in vitro were investigated in bFGF by 3H-TdR incorporation.Result：The results of 3H-TdR incorporation showed that 20 ng/mL～60 ng/mL bFGF significantly accelerated DNA synthesis of human pulp and periodontal ligament fibroblasts in vitro.bFGF did not stimulate collagen protein synthesis of human pulp and periodontal ligament fibroblasts in vitro.Conclusion：These results suggested that bFGF may stimulate DNA and protein synthesis of of human pulp and periodontal ligament,and may play an important role in pulp and periodontal tissue wound healing.