扶正解毒颗粒对染镍大鼠肾细胞DNA单链断裂的影响

Effects of Fuzheng Jiedu Granula on Nickel-induced DNA Damage in Rrats Renal Cells

目的研究扶正解毒颗粒(FJG)对镍(NiSO4)诱发大鼠肾细胞DNA损伤的拮抗作用。方法以Wistar大鼠NiSO4[2.5 mg/(kg.d)]染毒造模,FJG灌胃处理,分别以单细胞凝胶电泳(SCGE)技术及比色法检测肾组织细胞DNA单链断裂及总抗氧化力(TAC)。结果与NiSO4组相比,FJG各组"彗星"样细胞出现率(计数一定量细胞中彗星样细胞所占的比例)、尾长、Olive尾矩、尾DNA%、尾长/头长比均明显降低(P<0.05和P<0.01),TAC则显著升高(P<0.05和P<0.01)。结论 FJG对镍致细胞DNA损伤有一定的拮抗作用,其机制与抑制镍诱导氧化损伤有关。

Objective To study the effect of Fuzheng Jiedu Granula（FJG）on DNA damage in rats renal cells induced by Nickel sulfate（NiSO4）.Methods Rats nephrotoxicity was induced by intraperitoneal injection of NiSO4[2.5 mg/（kg·d）] and different dosages of FJG [5,10,20 mg/（kg·d）]were administered by gavage.Cellar DNA single strand break（SSB） and total antioxidant capacity（TAC） of renal tissues were determined with single cell gel-electrophoresis（SCGE） assay and colorimetry,respetively.Results The percentage of comet-like tail cells,length of tail,tail Olive moment,tail DNA percentage and ratio of tail/head were increased as well as TAC was increased significantly in FJG groups,compered with those in NiSO4 group（P0.05,P0.01）.Conclusion By inhibiting oxidative damage,FJG could play in antagonistic effect on DNA damage induced by nickel.