摘要
本研究采用过氧化物酶染色法检测380 名男子射出精液中白细胞( WBC) 数量。其中40 名精液正常生育者中无一例精液WBC增多;340 例不育者中精液WBC增多者有36 例,占10.59 % 。采用Western Blot 和图象分析法检测26 名男子精浆中白介素8(IL8) 的表达量发现,不育组精液WBC增多者其IL8 表达量明显高于不育组精液WBC未增多者和精液正常生育者(P< 0.01),表明IL8 的表达量与精液WBC数呈正相关。
Human semen from 380 men was examined for quantitating of leukocyte number using peroxidase stain.The increased leukocyte (WBC) number was not found in the semen of 40 normal fertile men.36 cases from 340 infertile patients had an increased WBC number(over 1×10 6/ml) in their semen.The proportion was up to 10.5%.Seminal plasma from 26 men was examined for evaluating the levels of IL 8 expression using both Western Blot and image analysis techniques.The level of IL*.8 expression from infertile seminal plasma with increased WBC number (881.22±323.68) was significantly higher than that of same group without increased WBC number (553.07±160.24) and that of normal fertile group (430.29±218.73).These results indicate that there was a positive correlation between IL 8 expression and WBC number in semen.
出处
《中国男科学杂志》
CAS
CSCD
1999年第4期227-229,共3页
Chinese Journal of Andrology