血管生成素-2与血管生成素-1比值在大鼠光气急性肺损伤中的变化

The changes of the ratio of angiopoietin-2 to angiopoietin-1 in the acute lung injury induced by phosgene in rats

目的观察吸入光气诱导的急性肺损伤大鼠血清中血管生成素（ang）-2、ang-1和ang-1／ang-2比值变化及其与肺损伤指标的关系。方法84只大鼠随机分为13个光气染毒组（光气组）和1个空气对照组，每组6只。观察不同时间后，用酶联免疫吸附（ELISA）法检测血清中ang-1、ang-2水平及肺泡灌洗液（BALF）中白细胞计数、总蛋白浓度。结果随着时间延长，光气组大鼠ang-2、肺湿／干重比值、BALF中白细胞计数、总蛋白浓度呈升高趋势，ang-1有降低趋势，血清中ang-2／ang-1比值呈升高趋势。6、8、10、12、14、16、18、20、22、24、48h光气组ang-2／ang-1比值明显高于空气对照组，差异有统计学意义（P〈0．01，P〈0．05）。ang-2与肺脏湿／干重比值、BALF中总蛋白浓度、BALF中白细胞计数呈正相关（栅直分别为0．441、0．283和0．439），差异有统计学意义（P〈0．01）。ang-1与肺脏湿／干重比值、BALF中自细胞计数的相关系数分别为-0．286和-0．362，差异有统计学意义（P〈0．01）。ang-2／ang-1比值与肺脏湿／干重比值、BALF中总蛋白浓度、BALF白细胞计数呈正相关（r值分别为0．683、0．534、0．710），差异有统计学意义（P〈0．01）。结论光气染毒后，大鼠血清中ang-2／ang-1比值与肺脏损伤指标呈正相关，可能可以作为判定急性肺脏损伤严重程度的早期重要指标。

Objective To observe the relationship between the indicators of lung injury and the ratio of angiopoietin-2 （ang-2） to angiopoietin-1 （ang-1） in serum of rats with acute lung injury induced by phosgene. Methods Eighty four aduh male SD rats were randomly divided into 13 phosgene groups and 1 control group. Control group was exposed to fresh air for 5 minutes; the phosgene groups were exposed to phosgene （ 100% purity of 8.33 mg/L phosgene ） for 5 minutes. At different time points after exposure, the wet/dry weight ratio of mouse lungs was examined, the levels of ang-1 and ang-2 in serum of rats were detected with ELISA assay, and the white blood cell count and total protein levels in bronchoalveolar lavage fluid （BALF） of rats were measured. Results In 48 h after exposure,the serum ang-2, lung wet/dry weight ratio, BALF white blood cell count, protein concentration tended to increase with time, and the serum ang-1 tended to decrease with time. The ratios of ang-2 to ang-1 at 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 and 48 h after exposure in phosgene groups were significantly higher than that in control group （P〈0.05 or P〈0.01）. There were the positive correlations between ang-2 and lung wet/dry ratio, BALF white blood cell count or protein concentration （P〈O.01）. There were the negative correlations between ang-1 and lung wet/dry ratio or BALF white blood cell count （P〈0.01）. There were the positive correlations between the ratio of ang-2 to ang-1 and lung wet/dry ratio, BALF white blood cell count or protein concentration （P〈0.01）. Conclusion After exposure of rats to phosgene, there is a positive correlation between the ratio of ang-2 to ang-1 and lung injury indicators, which may be an early indicator of the severity of phosgene-induced acute lung injury.