鸭胚卵内注射rhIGF-1对其出壳后卵泡抑素基因表达及胸肌发育的影响

The Effect of in ovo Injecting rhIGF-1 into Egg on the Expression of Follistatin Gene after out of Shell and the Muscle Development in Duck

本研究通过卵内注射技术,注射120、100、80和0(对照组)ng.mL-1的重组人胰岛素样生长因子(rhIGF-1)到孵化中的鸭胚蛋白中,并检测2日龄雏鸭胸肌发育和卵泡抑素(FST)表达的情况,探讨不同浓度rhIGF-1对FST表达及鸭胸肌发育的影响。结果表明,卵内注射不同浓度的rhIGF-1对雏鸭体质量的影响不显著(P>0.05);但雏鸭胸肌质量、胸肌率随rhIGF-1注射浓度的增加而升高,120 ng.mL-1处理组胸肌质量、胸肌率相对对照组有显著差异(P<0.05);120 ng.mL-1的rhIGF-1处理组肌纤维直径和横切面积最大,显著高于对照组(P<0.05);FST基因在雏鸭胸肌中的表达水平也随rhIGF-1的浓度增大而升高,其中以120 ng.mL-1处理组FST基因表达量最高,显著高于其它各处理组(P<0.05)。结果表明IGF-1对FST的调控作用以浓度依赖的方式进行,高浓度的IGF-1导致FST高表达并促进肌肉发育。

In order to investigate the effect of different IGF-1 concentrations on the expression of Follistatin（FST）,the different concentration rhIGF-1 with 120,100,80 and 0 ng·mL-1（the control group） were injected into the duck egg using in ovo injecting method,and then the expression of FST was detected using QPCR technique.The results showed that the body weight of ducklings at the age of two days among different treatment groups displayed no significant difference（P0.05）;while the breast muscle weight and breast muscle rate increased with the concentration of rhIGF-1 raising,and it displayed a significant difference between the 120 ng·mL-1 and the control groups（P0.05）.The diameter and cross section area of muscle fibers in 120 ng·mL-1 rhIGF-1 treatment were the most maximum with a significant difference comparing to control（P0.05）.The expression level of the FST in duckling breast muscle increased as the concentration of rhIGF-1 was raised,and the expression level of FST reached its highest in 120 ng·mL-1 rhIGF-1 treatment group with a significant difference comparing with other groups（P0.05）.After analyzing,the expression of FST is induced by IGF-1 in a dose-dependent manner,and the higher concentration of IGF-1 also promote duck breast muscle development.