钙离子超载在镉致大鼠肝细胞凋亡中的作用

Cadmium Induced Apoptosis in Rat Hepatocytes by Calcium Overloading

拟探讨Ca2+超载在镉诱导大鼠肝细胞凋亡中的作用。采用两步灌流法获得大鼠肝细胞,经过24 h培养,用醋酸镉、醋酸镉与Bapta-AM共同处理肝细胞。用MTT法检测细胞存活率,倒置显微镜和荧光显微镜观察细胞形态和凋亡,流式细胞仪检测细胞内Ca2+浓度、ROS生成和ΔΨm变化。结果表明,随着镉剂量的增加和作用时间的延长,细胞变形,坏死细胞和凋亡细胞增多,镉可极显著提高细胞内Ca2+浓度和ROS生成量(P<0.01),极显著降低线粒体膜电位(P<0.01),Bapta-AM可显著(P<0.05)或极显著(P<0.01)降低镉的毒性。研究结果提示Ca2+超载在镉致肝细胞凋亡中发挥重要作用。

The aims of this study were to explore the effect of i on the apoptosis of rat hepatocytes induced by cadmium.Rat hepatocytes were isolated by a two-step perfusion technique.After 24 h planting,hepatocytes were treated with cadmium in the presence or absence of Bapta-AM.Cell viability was evaluated with a colorimetric using MTT level.Cell morphology and apoptosis were photographed under inverted and fluorescence microscope.i,ROS generation and ΔΨm collapse were evaluated with flow cytometry.The results showed sharp deformation,necrosis and apoptosis in hepatocytes with the dose of Cd increased and time prolonged.Cd induced i increased,ΔΨm collapse and ROS generation（P0.01）.Bapta-AM effectively inhibited Cd-induced cytotoxicity（P0.05 or P0.01）.It was showed that cadmium induced apoptosis in rat hepatocytes by calcium overloading.