抑菌圈自动测量系统及其在抗生素效价评价中的应用

Inhibition Zone Automatic Measurement System and Its Application in Antibiotic Potency Evaluation

当前,国内外对抗生素测定的通用方法是微生物检定法,该方法是通过测量微生物抑菌圈的大小得到抗生素的效价。本文系统介绍了抑菌圈自动测量系统的硬件和软件设计,将图像形态处理学的理论应用于抑菌圈边缘的提取。应用各种边缘提取算子对抑菌圈边缘进行提取,发现应用canny算子得到的抑菌圈边缘较为理想。最后利用抗生素浓度与抑菌圈直径或面积的关系,求出样品的效价。

Nowadays,the general method for determination of antibiotics in domestic and abroad is microbial assays.The method is measuring the size of inhibition zone by microbial potency of antibiotics.This paper introduces the inhibition zone automatic measurement system hardware and software design,forms the image processing theories applied to extract the edge of inhibition zone.Using lots of edge detection operators to extract the edge of inhibition zone,we found that the canny operators are ideal application to extract edge of inhibition zone.Finally,use the relationship between concentration of antibiotics and inhibition zone diameter or area to obtain the potency of the samples.